Q8TWY1 (SYA_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	MK0900

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	915 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAM02113.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 915	915	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075265
Sites
	Metal binding	605	1	Zinc By similarity
	Metal binding	609	1	Zinc By similarity
	Metal binding	709	1	Zinc By similarity
	Metal binding	713	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TWY1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 6DD88BA1939CB4EE	FASTA	915	103,908
	10 20 30 40 50 60 MKVDPSEFEL EFFEEIGFER KRCPECGEYF WGPPEAEVCN ETPCVEYSFI GDPPASVKLD 70 80 90 100 110 120 VWEAGEEFFR FFERHDHEVL DRYPVVARWR DDIHLTIASI ACFQPWVTSG EVPPPANPLV 130 140 150 160 170 180 INQPCIRLND IDNVGRTGRH FTLFHMGGHH AFNNHPHDRR DIYWKEETVR LCYEFTVEKL 190 200 210 220 230 240 GIPEEKIAFK ESWWEGGGNA GPCFEVVVDG LELATLVFMQ YEQVGGEYRE LPQKIVDTGY 250 260 270 280 290 300 GIERYAWITT GEPTAYDAVF GDLVDATARD LGVEIDGEAR EILGELARVA GLMDVETESD 310 320 330 340 350 360 LRVLRNRVAR RLDLDVNELV RVAEPVEFVY GILDHARCLA FMLGDGVVPS NAGEGYLARL 370 380 390 400 410 420 VIRRALRLLD GLDAEREYLL EVVERVLEDL RGTYPELAER EEYIQDALEC EIDRYTRALK 430 440 450 460 470 480 RGKKEVRKRL EEKGELSFED LVELYDSHGI PPEVAREIAE DEGVEVEVPD DFYSRVAERH 490 500 510 520 530 540 EGPEEVEEGL EELERIAVEE ELPETELAFY DDEKRLEFKA EVIGTYEVNG DAWVVLDRTY 550 560 570 580 590 600 FYPEGGGQEA DRGTMRWKDG EAEVKDVQKV RGVVFHRIDG DVPPEGAEVE CEVDGERRMR 610 620 630 640 650 660 LTRNHTATHV ILEAARRVLG DHVWQAGAHK STDEARLDVT HHRRISDEEL REIERLANEI 670 680 690 700 710 720 VMKDLPVNKR FMDRNEAERR YGFELYQGGV VPGREIRVVE IEGWNVQACA GTHCDSTGEI 730 740 750 760 770 780 GPIKIVGRER IQDGVERIRF AAGEAALERI WETEDLLRET CEVLRVNPEN LPKTVKRFFE 790 800 810 820 830 840 EWKEQRKRIE RLERELVEAK LRAAPAEGRR VGDFTVTLVE LEDVEVGSVA GTVEELVKEH 850 860 870 880 890 900 ENLVLVAKIV SNGSCQVVVG SGESAPPAGE LMREIGKLIE GGGGGDERLA QGGGRNPDGL 910 TEDRLVEIVE DLAGG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02113.1. Different initiation.
RefSeq	NP_614183.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TWY1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477001.
GenomeReviews	Gene locus MK0900 in contig AE009439_GR.
KEGG	mka:MK0900.
NMPDR	fig|190192.1.peg.896.
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q8TWY1.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0900-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METKA

Accession

Primary (citable) accession number: Q8TWY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	August 2, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program