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Protein

tRNA(cytosine(8)) deaminase

Gene

MK0935

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deamimation of cytosine to uracil at position 8 of tRNA in 30 different tRNAs. This editing guarantees the proper folding and functionality of the tRNAs.1 Publication

Catalytic activityi

Cytosine8 in tRNA + H2O = uracil8 in tRNA + NH3.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc; catalytic1 Publication1
Active sitei46Proton donorBy similarity1
Metal bindingi67Zinc; catalytic1 Publication1
Metal bindingi70Zinc; catalytic1 Publication1

GO - Molecular functioni

  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • tRNA binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cytidine to uridine editing Source: UniProtKB
  • tRNA modification Source: UniProtKB

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding
Biological processtRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.35. 3274.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(cytosine(8)) deaminase (EC:3.5.4.35)
Alternative name(s):
Cytidine deaminase acting on tRNA base C8
Short name:
CDAT8
Gene namesi
Ordered Locus Names:MK0935
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004225951 – 278tRNA(cytosine(8)) deaminaseAdd BLAST278

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi190192.MK0935.

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Beta strandi8 – 11Combined sources4
Helixi12 – 18Combined sources7
Beta strandi25 – 30Combined sources6
Beta strandi33 – 39Combined sources7
Beta strandi41 – 43Combined sources3
Helixi45 – 52Combined sources8
Beta strandi59 – 64Combined sources6
Helixi68 – 75Combined sources8
Turni76 – 78Combined sources3
Beta strandi81 – 86Combined sources6
Helixi92 – 97Combined sources6
Turni98 – 100Combined sources3
Beta strandi102 – 108Combined sources7
Beta strandi121 – 125Combined sources5
Helixi134 – 147Combined sources14
Beta strandi151 – 157Combined sources7
Helixi158 – 160Combined sources3
Beta strandi164 – 172Combined sources9
Helixi174 – 184Combined sources11
Beta strandi188 – 192Combined sources5
Beta strandi194 – 198Combined sources5
Helixi201 – 211Combined sources11
Helixi213 – 215Combined sources3
Beta strandi218 – 228Combined sources11
Helixi235 – 247Combined sources13
Turni248 – 250Combined sources3
Beta strandi255 – 257Combined sources3
Beta strandi259 – 267Combined sources9
Beta strandi270 – 277Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G8QX-ray2.40A/B/C/D1-278[»]
ProteinModelPortaliQ8TWU6.
SMRiQ8TWU6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TWU6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 102CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST102
Domaini175 – 277THUMPPROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00056. Archaea.
ENOG4102TFP. Archaea.
COG1818. LUCA.
COG2131. LUCA.
KOiK16930.

Family and domain databases

InterProiView protein in InterPro
IPR002125. CMP_dCMP_dom.
IPR016193. Cytidine_deaminase-like.
IPR004114. THUMP_dom.
PfamiView protein in Pfam
PF00383. dCMP_cyt_deam_1. 1 hit.
PF02926. THUMP. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiView protein in PROSITE
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
PS51165. THUMP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TWU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSLAGQTV DVKKILNEIP KRTVTAALLE GGEIVAVEEA DDEHAERKLV
60 70 80 90 100
RRHDVEGKVV FVTARPCLYC ARELAEAGVA GVVYLGRGRG LGPYYLARSG
110 120 130 140 150
VEVVEVHPDE PLGYDPVDRL DVLLTFGGNP YLTEEDVAAR VYCLLTGRGF
160 170 180 190 200
DADIAPAPEN LSGRVEIMVT RGDPDEAVEL LKEELPVFRI RRFLISGEFD
210 220 230 240 250
RDELRERILE DIEPRILDPF AVRARIARAG AFSSSREAEV FIGDVLTSVG
260 270
REVNLNDPRT VVTVDVLGPR VSVGVEKR
Length:278
Mass (Da):30,618
Last modified:June 1, 2002 - v1
Checksum:i6F04884057684B4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02148.1.

Genome annotation databases

EnsemblBacteriaiAAM02148; AAM02148; MK0935.
KEGGimka:MK0935.

Entry informationi

Entry nameiCDAT8_METKA
AccessioniPrimary (citable) accession number: Q8TWU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: June 1, 2002
Last modified: June 7, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families