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Q8TWN3 (DNLI_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:MK0999
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559DNA ligase HAMAP-Rule MF_00407
PRO_0000059605

Sites

Active site2501N6-AMP-lysine intermediate By similarity
Binding site2481ATP By similarity
Binding site2551ATP By similarity
Binding site2701ATP By similarity
Binding site3001ATP By similarity
Binding site3411ATP By similarity
Binding site4171ATP By similarity
Binding site4231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TWN3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8C810526CC76402A

FASTA55963,337
        10         20         30         40         50         60 
MYYSSLAEAF ERLERISSRK AKISLIAQFL RQCPEDVVDT VALFLANQVF PGWDPRDLGI 

        70         80         90        100        110        120 
GSKLMRKVIA TATGSTDSEV TELFKRLGDL GLTAEELLKR RKTSTLLDSR PLMVGEVRET 

       130        140        150        160        170        180 
FEKIAEVEGE GAVKRKMRLM MGLLARAKPK EARYLVRQAL SELRTGVRES TVEEAIAQAF 

       190        200        210        220        230        240 
GVSRKLVERA HMLSNDLGLV AKVAMTKGEE GLREIDLRPM RPIKPMLAQA ARNVKEALAE 

       250        260        270        280        290        300 
VGGKGAVEIK LDGARVQVHS DGEEVRVYTR RIEDVTHALP DIVEAVKDCV DADEFILEGE 

       310        320        330        340        350        360 
AVAINPETGK PRPFQELLHR IKRKYDIEEV RKEIPVELHL FDCLYVDGES LVDTPFRERR 

       370        380        390        400        410        420 
RRLEEIVRER EGEVMLVEQV ITDDPKEAAE MFHRALEMGH EGVMVKDLDA NYTPGVRGKK 

       430        440        450        460        470        480 
MLKVKPVLET LDCVVIGGIW GKGKRKGLIG SYLLAVWDEN KENLLEVGKV GTGMDDETLE 

       490        500        510        520        530        540 
RLTKMFEDLI VEESGREVRF KPEVVFEVEF EDIQKSPKYS SGFALRFPRL VRVRDDLGPE 

       550 
DADTIEKVRR IYEEVLQKH 

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM02212.1.
RefSeqNP_614282.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TWN3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK0999.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM02212; AAM02212; MK0999.
GeneID1477100.
KEGGmka:MK0999.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAARVQVHK.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METKA
AccessionPrimary (citable) accession number: Q8TWN3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families