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Protein

DNA ligase

Gene

lig

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248ATPUniRule annotation1
Active sitei250N6-AMP-lysine intermediateUniRule annotation1
Binding sitei255ATPUniRule annotation1
Binding sitei270ATPUniRule annotation1
Binding sitei300ATPUniRule annotation1
Binding sitei341ATPUniRule annotation1
Binding sitei417ATPUniRule annotation1
Binding sitei423ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:MK0999
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596051 – 559DNA ligaseAdd BLAST559

Interactioni

Protein-protein interaction databases

STRINGi190192.MK0999

Structurei

3D structure databases

ProteinModelPortaliQ8TWN3
SMRiQ8TWN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TWN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYSSLAEAF ERLERISSRK AKISLIAQFL RQCPEDVVDT VALFLANQVF
60 70 80 90 100
PGWDPRDLGI GSKLMRKVIA TATGSTDSEV TELFKRLGDL GLTAEELLKR
110 120 130 140 150
RKTSTLLDSR PLMVGEVRET FEKIAEVEGE GAVKRKMRLM MGLLARAKPK
160 170 180 190 200
EARYLVRQAL SELRTGVRES TVEEAIAQAF GVSRKLVERA HMLSNDLGLV
210 220 230 240 250
AKVAMTKGEE GLREIDLRPM RPIKPMLAQA ARNVKEALAE VGGKGAVEIK
260 270 280 290 300
LDGARVQVHS DGEEVRVYTR RIEDVTHALP DIVEAVKDCV DADEFILEGE
310 320 330 340 350
AVAINPETGK PRPFQELLHR IKRKYDIEEV RKEIPVELHL FDCLYVDGES
360 370 380 390 400
LVDTPFRERR RRLEEIVRER EGEVMLVEQV ITDDPKEAAE MFHRALEMGH
410 420 430 440 450
EGVMVKDLDA NYTPGVRGKK MLKVKPVLET LDCVVIGGIW GKGKRKGLIG
460 470 480 490 500
SYLLAVWDEN KENLLEVGKV GTGMDDETLE RLTKMFEDLI VEESGREVRF
510 520 530 540 550
KPEVVFEVEF EDIQKSPKYS SGFALRFPRL VRVRDDLGPE DADTIEKVRR

IYEEVLQKH
Length:559
Mass (Da):63,337
Last modified:June 1, 2002 - v1
Checksum:i8C810526CC76402A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM02212.1

Genome annotation databases

EnsemblBacteriaiAAM02212; AAM02212; MK0999
KEGGimka:MK0999
PATRICifig|190192.8.peg.1047

Entry informationi

Entry nameiDNLI_METKA
AccessioniPrimary (citable) accession number: Q8TWN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health