Q8TWN3 (DNLI_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MK0999

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	DNA ligase HAMAP-Rule MF_00407		PRO_0000059605
Sites
	Active site	250	1	N6-AMP-lysine intermediate By similarity
	Binding site	248	1	ATP By similarity
	Binding site	255	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	300	1	ATP By similarity
	Binding site	341	1	ATP By similarity
	Binding site	417	1	ATP By similarity
	Binding site	423	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TWN3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 8C810526CC76402A	FASTA	559	63,337
	10 20 30 40 50 60 MYYSSLAEAF ERLERISSRK AKISLIAQFL RQCPEDVVDT VALFLANQVF PGWDPRDLGI 70 80 90 100 110 120 GSKLMRKVIA TATGSTDSEV TELFKRLGDL GLTAEELLKR RKTSTLLDSR PLMVGEVRET 130 140 150 160 170 180 FEKIAEVEGE GAVKRKMRLM MGLLARAKPK EARYLVRQAL SELRTGVRES TVEEAIAQAF 190 200 210 220 230 240 GVSRKLVERA HMLSNDLGLV AKVAMTKGEE GLREIDLRPM RPIKPMLAQA ARNVKEALAE 250 260 270 280 290 300 VGGKGAVEIK LDGARVQVHS DGEEVRVYTR RIEDVTHALP DIVEAVKDCV DADEFILEGE 310 320 330 340 350 360 AVAINPETGK PRPFQELLHR IKRKYDIEEV RKEIPVELHL FDCLYVDGES LVDTPFRERR 370 380 390 400 410 420 RRLEEIVRER EGEVMLVEQV ITDDPKEAAE MFHRALEMGH EGVMVKDLDA NYTPGVRGKK 430 440 450 460 470 480 MLKVKPVLET LDCVVIGGIW GKGKRKGLIG SYLLAVWDEN KENLLEVGKV GTGMDDETLE 490 500 510 520 530 540 RLTKMFEDLI VEESGREVRF KPEVVFEVEF EDIQKSPKYS SGFALRFPRL VRVRDDLGPE 550 DADTIEKVRR IYEEVLQKH

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02212.1.
RefSeq	NP_614282.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TWN3.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK0999.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM02212; AAM02212; MK0999.
GeneID	1477100.
KEGG	mka:MK0999.
Phylogenomic databases
eggNOG	COG1793.
HOGENOM	HOG000036008.
KO	K10747.
OMA	ARVQVHK.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00407. DNA_ligase.
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METKA

Accession

Primary (citable) accession number: Q8TWN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program