Q8TWG5 (MDH_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	MK1069

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: InterPro L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113486
Regions
	Nucleotide binding	8 – 14	7	NAD By similarity
	Nucleotide binding	121 – 123	3	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	98	1	NAD By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TWG5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D2AC04B7C0B6C26E	FASTA	317	34,609
	10 20 30 40 50 60 MSKVAVIGAT GRVGSTAAAR LALLDCVNEV TLIARPKSVD KLRGLRRDIL DSLAAAQKDA 70 80 90 100 110 120 EITIGCERDD YVDADVIVMT AGIPRKPGQT RLDLTKDNAA IIKKYLEGVA EENPEAIVLV 130 140 150 160 170 180 VTNPVDVLTY VALKVSGLPK NRVIGLGTHL DSMRFKVLIA KHFNVHMSEV HTRIIGEHGD 190 200 210 220 230 240 TMVPVISSTS VGGIPVTRMP GWEDFDVEEA VREVKEAGQR IIETWGGSQF GPAQAITNLV 250 260 270 280 290 300 RTILQDERRV LTVSAYLDGE IDGIRDVCIG VPARLGREGV LEIVPIELEE DEMRAFRRSV 310 KVVKEATREA MEAISER

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02282.1.
RefSeq	NP_614352.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TWG5.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK1069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM02282; AAM02282; MK1069.
GeneID	1477170.
KEGG	mka:MK1069.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213793.
KO	K00024.
OMA	DVASDKL.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_METKA

Accession

Primary (citable) accession number: Q8TWG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program