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Q8TWG3

- PUR2_METKA

UniProt

Q8TWG3 - PUR2_METKA

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Protein

Phosphoribosylamine--glycine ligase

Gene
purD, MK1071
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Magnesium or manganese 1 By similarity
Metal bindingi295 – 2951Magnesium or manganese 1 By similarity
Metal bindingi295 – 2951Magnesium or manganese 2 By similarity
Metal bindingi297 – 2971Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi139 – 20264ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene namesi
Name:purD
Ordered Locus Names:MK1071
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Phosphoribosylamine--glycine ligaseUniRule annotationPRO_0000151511Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190192.MK1071.

Structurei

3D structure databases

ProteinModelPortaliQ8TWG3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 325214ATP-graspAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033464.
KOiK01945.
OMAiFIDEYDG.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TWG3-1 [UniParc]FASTAAdd to Basket

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MLKVLLVGSG AREHAIAEAL CGAPDEDVEL YAFMGNRNPG IIRLAEDYVV    50
GDPTDVEAVA RAAADWNVDF AVVGPEDPLA EGVADRLEEE GIPTFGPKRG 100
PARIEWDKGF ARELMEKYDI PGRPEFGIFE DPDEACDFID ELGKPVAVKP 150
AGLTGGKGVK VVGDQLKNLD EAKEYVKEIF EEDIGGIPKV IIEEKCVGEE 200
YTIQAYTDGE KVIPTPAVQD HPHAYEGDKG PITGGMGSYS CPDGLLPFIT 250
KEDYERSVEI LERTVEAIKK ETGEPYRGVL YGQFMLTAEG PVVIEFNCRY 300
GDPEAMNILP IAEGDIVTLH ASIAEGSMEG EIEFLEKATV CKYVVPEGYP 350
ESSEGEGDVI EVDEECIHRY DAVPYYASVN LDEDGKIRMT SSRALAIVGI 400
GDELEQAEEA AESAIRECVS GERIRHRSDI GKHETVEKRV RRMKRIRGE 449
Length:449
Mass (Da):49,275
Last modified:June 1, 2002 - v1
Checksum:iFFD9F9189C9ADC48
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM02284.1.
RefSeqiNP_614354.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM02284; AAM02284; MK1071.
GeneIDi1477172.
KEGGimka:MK1071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM02284.1 .
RefSeqi NP_614354.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TWG3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK1071.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM02284 ; AAM02284 ; MK1071 .
GeneIDi 1477172.
KEGGi mka:MK1071.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033464.
KOi K01945.
OMAi FIDEYDG.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiPUR2_METKA
AccessioniPrimary (citable) accession number: Q8TWG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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