Q8TWG3 (PUR2_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	MK1071

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151511
Regions
	Domain	112 – 325	214	ATP-grasp
	Nucleotide binding	139 – 202	64	ATP By similarity
Sites
	Metal binding	283	1	Magnesium or manganese 1 By similarity
	Metal binding	295	1	Magnesium or manganese 1 By similarity
	Metal binding	295	1	Magnesium or manganese 2 By similarity
	Metal binding	297	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TWG3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: FFD9F9189C9ADC48	FASTA	449	49,275
	10 20 30 40 50 60 MLKVLLVGSG AREHAIAEAL CGAPDEDVEL YAFMGNRNPG IIRLAEDYVV GDPTDVEAVA 70 80 90 100 110 120 RAAADWNVDF AVVGPEDPLA EGVADRLEEE GIPTFGPKRG PARIEWDKGF ARELMEKYDI 130 140 150 160 170 180 PGRPEFGIFE DPDEACDFID ELGKPVAVKP AGLTGGKGVK VVGDQLKNLD EAKEYVKEIF 190 200 210 220 230 240 EEDIGGIPKV IIEEKCVGEE YTIQAYTDGE KVIPTPAVQD HPHAYEGDKG PITGGMGSYS 250 260 270 280 290 300 CPDGLLPFIT KEDYERSVEI LERTVEAIKK ETGEPYRGVL YGQFMLTAEG PVVIEFNCRY 310 320 330 340 350 360 GDPEAMNILP IAEGDIVTLH ASIAEGSMEG EIEFLEKATV CKYVVPEGYP ESSEGEGDVI 370 380 390 400 410 420 EVDEECIHRY DAVPYYASVN LDEDGKIRMT SSRALAIVGI GDELEQAEEA AESAIRECVS 430 440 GERIRHRSDI GKHETVEKRV RRMKRIRGE

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02284.1.
RefSeq	NP_614354.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TWG3.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK1071.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM02284; AAM02284; MK1071.
GeneID	1477172.
KEGG	mka:MK1071.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033464.
KO	K01945.
OMA	EPLFAGV.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METKA

Accession

Primary (citable) accession number: Q8TWG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program