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Q8TWG3 (PUR2_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:MK1071
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151511

Regions

Domain112 – 325214ATP-grasp
Nucleotide binding139 – 20264ATP By similarity

Sites

Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2951Magnesium or manganese 1 By similarity
Metal binding2951Magnesium or manganese 2 By similarity
Metal binding2971Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TWG3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: FFD9F9189C9ADC48

FASTA44949,275
        10         20         30         40         50         60 
MLKVLLVGSG AREHAIAEAL CGAPDEDVEL YAFMGNRNPG IIRLAEDYVV GDPTDVEAVA 

        70         80         90        100        110        120 
RAAADWNVDF AVVGPEDPLA EGVADRLEEE GIPTFGPKRG PARIEWDKGF ARELMEKYDI 

       130        140        150        160        170        180 
PGRPEFGIFE DPDEACDFID ELGKPVAVKP AGLTGGKGVK VVGDQLKNLD EAKEYVKEIF 

       190        200        210        220        230        240 
EEDIGGIPKV IIEEKCVGEE YTIQAYTDGE KVIPTPAVQD HPHAYEGDKG PITGGMGSYS 

       250        260        270        280        290        300 
CPDGLLPFIT KEDYERSVEI LERTVEAIKK ETGEPYRGVL YGQFMLTAEG PVVIEFNCRY 

       310        320        330        340        350        360 
GDPEAMNILP IAEGDIVTLH ASIAEGSMEG EIEFLEKATV CKYVVPEGYP ESSEGEGDVI 

       370        380        390        400        410        420 
EVDEECIHRY DAVPYYASVN LDEDGKIRMT SSRALAIVGI GDELEQAEEA AESAIRECVS 

       430        440 
GERIRHRSDI GKHETVEKRV RRMKRIRGE 

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM02284.1.
RefSeqNP_614354.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TWG3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK1071.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM02284; AAM02284; MK1071.
GeneID1477172.
KEGGmka:MK1071.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAENLECEH.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METKA
AccessionPrimary (citable) accession number: Q8TWG3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways