Q8TW12 (PUR5_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	MK1226

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_A.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A		PRO_0000148282

Sequences

	Sequence		Length	Mass (Da)
	Q8TW12 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 620F37A3B18DC964	FASTA	345	37,492
	10 20 30 40 50 60 MSEMTYKDAG VDIDKEAFAV RAIRDVLEKY KVEPEGCREV EGIGHYAAVL EVHGELLTLN 70 80 90 100 110 120 VDGVGSKVLV AQLVGRYDTV GIDAIAMNAN DAVCLGARPL AFLDYLAMED PDPDVCAEIA 130 140 150 160 170 180 EGLGKGAREA GAPIVGGELA TLPEVIRGKE EGRGFDLVVA CLGRVEGDPI TGEDVEPGDA 190 200 210 220 230 240 IVGLRSSGIH SNGLTLARKV LLSEYDVHDE LPHGRTVAEE LLEPTRIYVR PVMEVLRDYE 250 260 270 280 290 300 VRGIAHITGG GVENLKRLRD DVRYVLDDPF EPHPVFEIIR ELGNVPVEEM YRTFNMGMGM 310 320 330 340 ALIVPEEEAE DVVDTVSKHV EAKIVGHVEE GRGVVVHMDG HEVKL

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02439.1.
RefSeq	NP_614509.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TW12.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477821.
GenomeReviews	Gene locus MK1226 in contig AE009439_GR.
KEGG	mka:MK1226.
NMPDR	fig|190192.1.peg.1222.
Phylogenomic databases
HOGENOM	HBG531222.
OMA	PENIPRV.
PhylomeDB	Q8TW12.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1226-MONOMER.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_METKA

Accession

Primary (citable) accession number: Q8TW12

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program