Q8TW08 (G1PDH_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Synonyms:	gldA
Ordered Locus Names:	MK1230

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000157343
Regions
	Nucleotide binding	99 – 103	5	NAD By similarity
	Nucleotide binding	121 – 124	4	NAD By similarity
Sites
	Metal binding	173	1	Zinc; catalytic By similarity
	Metal binding	253	1	Zinc; catalytic By similarity
	Metal binding	269	1	Zinc; catalytic By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	130	1	NAD By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	257	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TW08 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 64A575C0A35AA30A	FASTA	353	37,642
	10 20 30 40 50 60 MSVPKKRMQL PREVVVGSNV LPEVPKLLRS VGVPDGVVAV FSGRTTMKIA GNEVADHLEE 70 80 90 100 110 120 AGYQTSPVIV KGSTGDDVKK ALEALDEIDA DVVAAVGGGK VIDVAKVASY RRGIPFISVP 130 140 150 160 170 180 TSASHDGIAS PFASIRREGR PYSEPAQAPL AILADIEVIR EAPERLIRAG VGDVVSNVTA 190 200 210 220 230 240 VKDWRLAHRL RNEPYSEYAS SLSLMAARIV MKNAKPIGKL LPEGIKKLVQ ALISGGVAMS 250 260 270 280 290 300 IAGSSRPCSG SEHLFSHALD VIAERPALHG EQCGVGTIIM EYLHGGNWRE IRETLETAGA 310 320 330 340 350 PTTAEDLGVS DEEIIEALCR AHKIRPDRYT ILGDKGLTRE AAERAAEETG VIQ

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02443.1.
RefSeq	NP_614513.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TW08.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477825.
GenomeReviews	Gene locus MK1230 in contig AE009439_GR.
KEGG	mka:MK1230.
NMPDR	fig|190192.1.peg.1226.
Phylogenomic databases
HOGENOM	HBG672951.
OMA	AIHGELV.
PhylomeDB	Q8TW08.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1230-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METKA

Accession

Primary (citable) accession number: Q8TW08

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program