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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MK1364
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Leucine--tRNA ligasePRO_0000152132Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190192.MK1364.

Structurei

3D structure databases

ProteinModelPortaliQ8TVM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 4611"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiTREFHTT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TVM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERPEERQW KEWEEAGLFE ADPDDRESVY ITVAYPYPSG SMHVGHARTY
60 70 80 90 100
LVPDIYARFK RMQGYNVLFP MAFHVTGTPV VGIAERIKEG DEDTIRLYRD
110 120 130 140 150
LYGVPEEELE KFTEPEAIVE YFAREYEENM KRMGYSIDWR RKFTTVDPEY
160 170 180 190 200
RSFITWQYLR LREKGLVDKG EHPVRYCPHC ENPVGDHDLL EGEDATIEEL
210 220 230 240 250
TLVKFPVEGD DLILVAATFR PETLYGATNV WVKPDEEYLV VEVDGERWVV
260 270 280 290 300
SEEAYRNLRH QKDGVEKVDT VRGEELIGES VVNPVTGEAL PVLPAEFIDP
310 320 330 340 350
KFGTGVVYSV PAHAPADAAA LEDLKKDPSV LEEYGVDPSV VEELEPVQVI
360 370 380 390 400
EVEGYGEFPA YDALEEHGIE SQTDPELEKA TQEVYRAELH KGVMVVDEFE
410 420 430 440 450
GTPVREAREE IKSRLIESGD ADVMYDFSEK PVICRCGTEC VVRILKDQWF
460 470 480 490 500
LRYSDGEWKE RAEELLGRME IVPEEVRANF EDTIEWLDDW ACARRVGLGT
510 520 530 540 550
PLPWDPDWIV EPLSDSTVYM AYYTIAHRLK GKGELPPEVF DYVFLGEGDP
560 570 580 590 600
EEIAEKAGLD VEELEAMREE FEYWYPLNWR LSAKDLVTNH LTFFIFHHAA
610 620 630 640 650
LFPEDKWPKG IVVFGMGLLE GQKMSSSKGN VVLLSEALDE YGPDVVRLFL
660 670 680 690 700
ATSAEPWQDF DWRDEYVRGV QRHLERFETL IRDHADESVE DKDAVDRWFL
710 720 730 740 750
HEFREVVEET TEALEGFQIR RAYNRAFYGV MKLLREYEAM KGHVKILGEI
760 770 780 790 800
AEDWLKLLHP VIPFATDRLW REVLGEDSFL LEEEWPDPSE YPEEPELSVA
810 820 830 840 850
KEVLDRLIED VRDVEKVIGA EPGYTLHVYL APEWQWRALE LILKDKEFGE
860 870 880 890 900
VMSELMKDEG LREKGDEVAK IVQELTKEDL PEDVDVDALR EALTEFLEAA
910 920 930 940
GRALTDKTGA SEVVIHTDPE EAPGPEDRKA GARPLRPGIW LEE
Length:943
Mass (Da):108,672
Last modified:June 1, 2002 - v1
Checksum:i9B5D4567D2588094
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02577.1.
RefSeqiNP_614647.1. NC_003551.1.
WP_011019732.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM02577; AAM02577; MK1364.
GeneIDi1477959.
KEGGimka:MK1364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02577.1.
RefSeqiNP_614647.1. NC_003551.1.
WP_011019732.1. NC_003551.1.

3D structure databases

ProteinModelPortaliQ8TVM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK1364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02577; AAM02577; MK1364.
GeneIDi1477959.
KEGGimka:MK1364.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiTREFHTT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiSYL_METKA
AccessioniPrimary (citable) accession number: Q8TVM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: May 27, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.