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Q8TVM4 (SYL_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MK1364
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152132

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif623 – 6275"KMSKS" region HAMAP-Rule MF_00049

Sequences

Sequence LengthMass (Da)Tools
Q8TVM4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9B5D4567D2588094

FASTA943108,672
        10         20         30         40         50         60 
MAERPEERQW KEWEEAGLFE ADPDDRESVY ITVAYPYPSG SMHVGHARTY LVPDIYARFK 

        70         80         90        100        110        120 
RMQGYNVLFP MAFHVTGTPV VGIAERIKEG DEDTIRLYRD LYGVPEEELE KFTEPEAIVE 

       130        140        150        160        170        180 
YFAREYEENM KRMGYSIDWR RKFTTVDPEY RSFITWQYLR LREKGLVDKG EHPVRYCPHC 

       190        200        210        220        230        240 
ENPVGDHDLL EGEDATIEEL TLVKFPVEGD DLILVAATFR PETLYGATNV WVKPDEEYLV 

       250        260        270        280        290        300 
VEVDGERWVV SEEAYRNLRH QKDGVEKVDT VRGEELIGES VVNPVTGEAL PVLPAEFIDP 

       310        320        330        340        350        360 
KFGTGVVYSV PAHAPADAAA LEDLKKDPSV LEEYGVDPSV VEELEPVQVI EVEGYGEFPA 

       370        380        390        400        410        420 
YDALEEHGIE SQTDPELEKA TQEVYRAELH KGVMVVDEFE GTPVREAREE IKSRLIESGD 

       430        440        450        460        470        480 
ADVMYDFSEK PVICRCGTEC VVRILKDQWF LRYSDGEWKE RAEELLGRME IVPEEVRANF 

       490        500        510        520        530        540 
EDTIEWLDDW ACARRVGLGT PLPWDPDWIV EPLSDSTVYM AYYTIAHRLK GKGELPPEVF 

       550        560        570        580        590        600 
DYVFLGEGDP EEIAEKAGLD VEELEAMREE FEYWYPLNWR LSAKDLVTNH LTFFIFHHAA 

       610        620        630        640        650        660 
LFPEDKWPKG IVVFGMGLLE GQKMSSSKGN VVLLSEALDE YGPDVVRLFL ATSAEPWQDF 

       670        680        690        700        710        720 
DWRDEYVRGV QRHLERFETL IRDHADESVE DKDAVDRWFL HEFREVVEET TEALEGFQIR 

       730        740        750        760        770        780 
RAYNRAFYGV MKLLREYEAM KGHVKILGEI AEDWLKLLHP VIPFATDRLW REVLGEDSFL 

       790        800        810        820        830        840 
LEEEWPDPSE YPEEPELSVA KEVLDRLIED VRDVEKVIGA EPGYTLHVYL APEWQWRALE 

       850        860        870        880        890        900 
LILKDKEFGE VMSELMKDEG LREKGDEVAK IVQELTKEDL PEDVDVDALR EALTEFLEAA 

       910        920        930        940 
GRALTDKTGA SEVVIHTDPE EAPGPEDRKA GARPLRPGIW LEE 

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM02577.1.
RefSeqNP_614647.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TVM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK1364.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM02577; AAM02577; MK1364.
GeneID1477959.
KEGGmka:MK1364.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIMNDLRW.
ProtClustDBPRK12300.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METKA
AccessionPrimary (citable) accession number: Q8TVM4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries