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Protein

3-isopropylmalate dehydratase large subunit 1

Gene

leuC1

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit 1 (leuC1), 3-isopropylmalate dehydratase small subunit 1 (leuD1), 3-isopropylmalate dehydratase large subunit 2 (leuC2), 3-isopropylmalate dehydratase small subunit 2 (leuD2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi298 – 2981Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi358 – 3581Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi361 – 3611Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunit 1UniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomerase 1UniRule annotation
Short name:
IPMI 1UniRule annotation
Isopropylmalate isomerase 1UniRule annotation
Gene namesi
Name:leuC1UniRule annotation
Ordered Locus Names:MK1440
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4184183-isopropylmalate dehydratase large subunit 1PRO_0000076870Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK1440.

Structurei

3D structure databases

ProteinModelPortaliQ8TVF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01698. Archaea.
COG0065. LUCA.
HOGENOMiHOG000226971.
KOiK01703.
OMAiMACAMAT.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
IPR006251. Homoacnase/IPMdehydase_lsu.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01343. hacA_fam. 1 hit.
TIGR02086. IPMI_arch. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TVF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTMAEKIL SRASGEDAEA GDIVVANIDV AMVHDITGPI TVQRLEEMGV
60 70 80 90 100
ERVWDPSKIV VLFDHQVPAD SVEAAENHKI MREFVEEQGI EHFYDVREGV
110 120 130 140 150
CHQVLPEKGH VRPGDVIVGA DSHTCTHGAL GAFATGIGST DMAAVFATGK
160 170 180 190 200
LWFRVPETYR VEITGELPEG VYAKDVVLKV TGEIGADGAT YMAIEYHGEV
210 220 230 240 250
VREMSVSDRM CLCNMAIEMG AKTGMVPPDE KTLEYVKKRA GTEGRPVEPD
260 270 280 290 300
PDARYEAELT LDVSDLEPQV AKPFSPDNVV PVGEVEGIAI DQVFIGSCTN
310 320 330 340 350
GRYEDLKVAA EVLEGEEVHD DVRLIVIPAS REVYHRTLKD GVLEVLHEAG
360 370 380 390 400
ALICPPNCGP CLGGHMGVLA EGERCVATSN RNFPGRMGHR ESEVYLASPA
410
TAAASAIEGE ITDPRPYL
Length:418
Mass (Da):45,202
Last modified:June 1, 2002 - v1
Checksum:iFABA735A9BD51C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02653.1.

Genome annotation databases

EnsemblBacteriaiAAM02653; AAM02653; MK1440.
KEGGimka:MK1440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02653.1.

3D structure databases

ProteinModelPortaliQ8TVF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK1440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02653; AAM02653; MK1440.
KEGGimka:MK1440.

Phylogenomic databases

eggNOGiarCOG01698. Archaea.
COG0065. LUCA.
HOGENOMiHOG000226971.
KOiK01703.
OMAiMACAMAT.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
IPR006251. Homoacnase/IPMdehydase_lsu.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01343. hacA_fam. 1 hit.
TIGR02086. IPMI_arch. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUC1_METKA
AccessioniPrimary (citable) accession number: Q8TVF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.