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Protein

3-isopropylmalate dehydratase large subunit 1

Gene

leuC1

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit 1 (leuC1), 3-isopropylmalate dehydratase small subunit 1 (leuD1), 3-isopropylmalate dehydratase large subunit 2 (leuC2), 3-isopropylmalate dehydratase small subunit 2 (leuD2)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi358Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi361Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunit 1UniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomerase 1UniRule annotation
Short name:
IPMI 1UniRule annotation
Isopropylmalate isomerase 1UniRule annotation
Gene namesi
Name:leuC1UniRule annotation
Ordered Locus Names:MK1440
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768701 – 4183-isopropylmalate dehydratase large subunit 1Add BLAST418

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK1440

Structurei

3D structure databases

ProteinModelPortaliQ8TVF2
SMRiQ8TVF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01698 Archaea
COG0065 LUCA
HOGENOMiHOG000226971
KOiK01703
OMAiFDHQVPA
OrthoDBiPOG093Z03G6

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01027 LeuC_type2, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR011826 HAcnase/IPMdehydase_lsu_prok
IPR006251 Homoacnase/IPMdehydase_lsu
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01343 hacA_fam, 1 hit
TIGR02086 IPMI_arch, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TVF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTMAEKIL SRASGEDAEA GDIVVANIDV AMVHDITGPI TVQRLEEMGV
60 70 80 90 100
ERVWDPSKIV VLFDHQVPAD SVEAAENHKI MREFVEEQGI EHFYDVREGV
110 120 130 140 150
CHQVLPEKGH VRPGDVIVGA DSHTCTHGAL GAFATGIGST DMAAVFATGK
160 170 180 190 200
LWFRVPETYR VEITGELPEG VYAKDVVLKV TGEIGADGAT YMAIEYHGEV
210 220 230 240 250
VREMSVSDRM CLCNMAIEMG AKTGMVPPDE KTLEYVKKRA GTEGRPVEPD
260 270 280 290 300
PDARYEAELT LDVSDLEPQV AKPFSPDNVV PVGEVEGIAI DQVFIGSCTN
310 320 330 340 350
GRYEDLKVAA EVLEGEEVHD DVRLIVIPAS REVYHRTLKD GVLEVLHEAG
360 370 380 390 400
ALICPPNCGP CLGGHMGVLA EGERCVATSN RNFPGRMGHR ESEVYLASPA
410
TAAASAIEGE ITDPRPYL
Length:418
Mass (Da):45,202
Last modified:June 1, 2002 - v1
Checksum:iFABA735A9BD51C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM02653.1

Genome annotation databases

EnsemblBacteriaiAAM02653; AAM02653; MK1440
KEGGimka:MK1440
PATRICifig|190192.8.peg.1596

Similar proteinsi

Entry informationi

Entry nameiLEUC1_METKA
AccessioniPrimary (citable) accession number: Q8TVF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health