Reviewed, UniProtKB/Swiss-Prot Q8TVA7 (MTRF_METKA)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit F
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F

Gene names

Name:	mtrF
Ordered Locus Names:	MK1485

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	75 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01099
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01099
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Miscellaneous	Unlike other orthologs, mtrF from M.kandleri is not located in the mtr operon. HAMAP MF_01099
Sequence similarities	Belongs to the mtrF family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 75	75	Tetrahydromethanopterin S-methyltransferase subunit F HAMAP MF_01099		PRO_0000147549
Regions
	Transmembrane	53 – 73	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TVA7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0186C402CCFCEF28	FASTA	75	8,130
	10 20 30 40 50 60 MAEEGSELKE VIIGAPAMAD TDRADTYVND VRDSSQFFGR DARLYFGLNV NRFAGLACGM 70 VFAGVLLVPL LLLAF

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010439 Genomic DNA. Translation: AAM02698.1.
RefSeq	NP_614768.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1478080.
GenomeReviews	Gene locus MK1485 in contig AE009439_GR.
KEGG	mka:MK1485.
NMPDR	fig|190192.1.peg.1481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TVA7.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1485-MON.
BRENDA	2.1.1.86. 7577.
Family and domain databases
HAMAP	MF_01099. [Tree]
ProtoNet	Search...

Entry information

Entry name

MTRF_METKA

Accession

Primary (citable) accession number: Q8TVA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)