Q8TV34 (DUT_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	MK1566

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635
Sequence similarities	Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635		PRO_0000153639

Sequences

	Sequence		Length	Mass (Da)
	Q8TV34 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 203B7FED196C8958	FASTA	154	17,315
	10 20 30 40 50 60 MILGEYVLRD LFPDLDEDQY QPAGIDLKLD RVFRLEGRGA LLEGDDKRLP EYREVETEGG 70 80 90 100 110 120 VFELEPNVPY VLELAPELEI PEDTAVLFLP RSTLLRSGVS VHTALGDPGF RGKIRVLAVN 130 140 150 HHAAPYRIAH GERVVQAVFL RAEDAGRYEG DYGR

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02779.1.
RefSeq	NP_614849.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TV34.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK1566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM02779; AAM02779; MK1566.
GeneID	1478161.
KEGG	mka:MK1566.
Phylogenomic databases
eggNOG	COG0717.
HOGENOM	HOG000228599.
KO	K01520.
OMA	QCGIDLR.
ProtClustDB	CLSK798221.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00635. dUTPase_arch.
InterPro	IPR008180. dUTP_pyroPase. IPR023537. dUTP_pyroPase_arch. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUT_METKA

Accession

Primary (citable) accession number: Q8TV34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program