Q8TUX2 (ARGB_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	MK1631

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_A
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_A
Subunit structure	Homodimer By similarity. HAMAP MF_00082_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_A.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Acetylglutamate kinase HAMAP MF_00082_A		PRO_0000112695
Regions
	Region	30 – 31	2	Substrate binding By similarity
Sites
	Binding site	52	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Site	5	1	Transition state stabilizer By similarity
	Site	214	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TUX2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D550515A74C373FB	FASTA	246	26,086
	10 20 30 40 50 60 MEVIKVGGEV LDRVEDLARV IDDSILVHGG GPEVSDVMER MGLEPRFVRG LRVTDRETLQ 70 80 90 100 110 120 VVMMVLAGLV NKRLVAELRS EGINALGLSG VDGGLLIAEK RSEVVDGEEV DLGYVGDVKR 130 140 150 160 170 180 VNAELLESLL DAGYVPVVAP LGAGEDGTVY NVNADTAAGA IAGAVRADRL VLLTDVPGVL 190 200 210 220 230 240 EDLDDPETLI ERVRPEDVEE LEEKGIVTGG MVPKLEAAKM AVEAGCREAV ITNLEGLLEG RGTIVR

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM02844.1.
RefSeq	NP_614914.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TUX2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1478226.
GenomeReviews	Gene locus MK1631 in contig AE009439_GR.
KEGG	mka:MK1631.
NMPDR	fig|190192.1.peg.1627.
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGCLVDD.
PhylomeDB	Q8TUX2.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1631-MONOMER.
Family and domain databases
HAMAP	MF_00082_A. ArgB_A. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_METKA

Accession

Primary (citable) accession number: Q8TUX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program