Reviewed, UniProtKB/Swiss-Prot Q8TUV3 (DP2L_METKA)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MK1650

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152576

Sequences

	Sequence		Length	Mass (Da)
	Q8TUV3-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4BEEE6117BCDD9AD	FASTA	1,240	140,145
	10 20 30 40 50 60 MNRAREELDR YRETLEEVSS RFVDVATKAR QRREDPKPEP EVMLATSIGE RVEGLLQVEN 70 80 90 100 110 120 VADRLEELEE ELGDREEATF RIVEEVIKGE LKVKGDLPLH KRIDYAVRIG LAVLTEAVVS 130 140 150 160 170 180 APLEGIAAVE IRERGTGHRV VDESEPPHEE PKLVCTECGK EVDPENCYLA VKYAGPIRAA 190 200 210 220 230 240 GGTAAALSAL LADYARQVAG LPRFNPDDFD HDLVGRYVEE VVTYLDKVGS FQYNPSEEEV 250 260 270 280 290 300 ELVAKNIPIE IDGEPTEEVE VQGHRDIPHL PNQLRGGALL VICEGICQKA PKLIKRVEKY 310 320 330 340 350 360 GIDGWEFLEK LVNKGSDDEE EGEEEKTKIK PNDKYMGELV AGRPLLSHPS AKGGFRLRYG 370 380 390 400 410 420 RARNTGFAAV GVHPSLMYVT KGFIVIGTQL KVERPGKAAC VLPVTEIEPP VVKLRDGSVV 430 440 450 460 470 480 RLDDPREAKE LVEKDEIEEI LDLGEMLVAV GEFIENNHPL VPPAYCPEWW VKEVPDVVKV 490 500 510 520 530 540 IGLRKNLPND VFEKLKDVPL KRLVKEASRL SGNGNNLDGF LNGPVKVARS IVELVRKEVI 550 560 570 580 590 600 PRLSSPERMS VEEAIELSRE YGVPFHPKYT FLWHDVKPKD VDELREALEV AGSEWGNLRV 610 620 630 640 650 660 EFENDGEIKR ILEDLLVPHR LEDDTIVVEE PWASALLAQL GYDPESGEFR EQDEDLDYLL 670 680 690 700 710 720 DYLVIRDETC RYVSKLAGFP IREKAPTRIG ARMGRPEKAR ERKMSPPPHV LFPIGIAGGN 730 740 750 760 770 780 QRDIMKFHRG ESEDTDRVEV CYRICPECDR LVPYRVCPFC GTETVQYCNR CDEPADECDC 790 800 810 820 830 840 EEPDPVVRAD IERNDDPYSS LPVRELVRRA EEEVGTTDTL KGVKGMTSRL KMPEPLQKGI 850 860 870 880 890 900 LRAKRDLFVF KDGTLRFDCN DCPLTHVRLK EVGLTPFKAR LLGFERDING DPVVSEDQVV 910 920 930 940 950 960 ELYPQDVVLP RKAAEWAVRV CQYLDDLLRK YYGLEPVYGV EKPEDLIGHL IVTLAPHTSC 970 980 990 1000 1010 1020 GVVGRVVGIA DINCWYNHPI INAARRRNCD GDEDAFMLLL DVLLNFSRLY LPDKRGGLMD 1030 1040 1050 1060 1070 1080 APLVLTAVVD PYEIDDEVWN MDVCGDYPLE LYRKALEYAD AGEAEELIER LEDRLDLPRG 1090 1100 1110 1120 1130 1140 LQFTHDTEAI DLGPTVTRYS RLEKMEEKLE EQLDLARRIR AVDESDVAKI VLDSHFLPDI 1150 1160 1170 1180 1190 1200 KGNLRKFGRQ KFRCSRCNAK FNVPPLSGKC PRCGGDVLLT IYPATATKYL EPAKRLVEEF 1210 1220 1230 1240 GTHDEEWRTI ASEVELLEEE AKTLFGSDHS VSLKKFFGET

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010454 Genomic DNA. Translation: AAM02863.1.
RefSeq	NP_614933.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1478245.
GenomeReviews	Gene locus MK1650 in contig AE009439_GR.
KEGG	mka:MK1650.
NMPDR	fig|190192.1.peg.1646.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TUV3.
OMA	Q8TUV3. NCDGDED.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1650-MON.
BRENDA	2.7.7.7. 7577.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METKA

Accession

Primary (citable) accession number: Q8TUV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	June 1, 2002
Last modified:	May 5, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)