Reviewed, UniProtKB/Swiss-Prot Q8TUT0 (VATB_METKA)

Names and origin

Protein names

Recommended name:
    V-type ATP synthase beta chain
Alternative name(s):
    V-ATPase subunit B
Cleaved into the following chain:
    1- Recommended name:
            Mka atpB intein

Gene names

Name:	atpB
Ordered Locus Names:	MK1673

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit. HAMAP MF_00310
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the ATPase alpha/beta chains family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Intron homing Ion transport Transport
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP
Cellular component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW hydrogen ion transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	V-type ATP synthase beta chain, 1st part Potential		PRO_0000002476
	Chain	261 – 777	517	Mka atpB intein Potential		PRO_0000002477
	Chain	778 – 990	213	V-type ATP synthase beta chain, 2nd part Potential		PRO_0000002478
Regions
	Domain	491 – 614	124	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q8TUT0-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7AC6BE8A32B31585	FASTA	990	110,354
	10 20 30 40 50 60 MAEAERPAGK EYTTISEVSG PLMVVEGVEG AKYGEVVEVE TPTGEVRRGQ VLEARRDAAV 70 80 90 100 110 120 VQVFEGTSGL DTTSTKVRFT GETLRIPVST DLLGRILNGR GEPIDGGPEI VPEDELDIHG 130 140 150 160 170 180 APINPAARKY PSDFIQTGIS AIDGMNTLVR GQKLPIFSGS GLPHNELAAQ IARQATVPGE 190 200 210 220 230 240 EEEFAVVFAA MGITHEEAAF FRREFEETGA LDRAVLILNL ADDPSMERII TPRIALTVAE 250 260 270 280 290 300 YLAFENDMHV LVILTDMTNY CFAPGTRVIT ASGDVVEIDE IVERAAETAV DGGLREGSTE 310 320 330 340 350 360 VTVGVTNVRT LAAWDGDLTS NDVVAVEKIE APSRAVRVRT RSGAELVVSE DHKFLVDTED 370 380 390 400 410 420 GPRMVEASEL KSGDELYSVR ELRVSEKVPT YLELLLEAED KFYVHPTEEF EEAVAERYGS 430 440 450 460 470 480 LAEACREKEL PYRAREAKER RYYELSEFAR LATAVIESVD EATEYIDYVT AGGRKRVKFS 490 500 510 520 530 540 SPRPGKEVMY VAGLIASDGS VDTERGFVMF SNTERELLSA FEEIVTEEFG VDASKTENQN 550 560 570 580 590 600 GVTMLRVNSR VLARVFERLA DPKTVLKMPR ELVAAYLAGY VDGDGHLKDG KIVITTADRE 610 620 630 640 650 660 RAGDLQLLLK RLGVPSVLRE RDGAYDVVVT GHDAAELAEE LPLRHPKKAE AAASMSSGRR 670 680 690 700 710 720 SSRFDRVSRR FGRLLREVRR KYGVRASDLG SSSTISQIES GERRATRRLA LEIVERLEEV 730 740 750 760 770 780 VGDVEEVREL RELAEGNYVL DEVVEVETVE YEHEYLYDVT VVPDHTLVVE NGIITSNCEA 790 800 810 820 830 840 LREISAAREE VPGRRGYPGY MYTDLATIYE RAGCIRGRKG SITQMPILTM PHDDITHPIP 850 860 870 880 890 900 DLTGYITEGQ IVLSRDLHRR GIYPPIDVLP SLSRLMDEGI GKGKTREDHP DLSNQLYAAY 910 920 930 940 950 960 AEGRDLRDLV AVVGEEALTE RDRKFLKFAD EFEQRFVKQG RDENRSIEET LDLGWELLAI 970 980 990 LPERELKRVS DELIEKYHPK YRQKKEEQEE

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009439 Genomic DNA. Translation: AAM02886.1.
RefSeq	NP_614956.1.
3D structure databases
SMR	Q8TUT0. Positions 765-981.
ModBase	Search...
Genome annotation databases
GeneID	1478268.
GenomeReviews	Gene locus MK1673 in contig AE009439_GR.
KEGG	mka:MK1673.
NMPDR	fig|190192.1.peg.1669.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	MKAN190192:MK1673-MON.
BRENDA	3.6.3.14. 7577.
Family and domain databases
HAMAP	MF_00310. [Tree]
InterPro	IPR018118. ATPase_F1/A1-cplx_a/bsu_N. IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C. IPR004100. ATPase_F1/V1/A1-cplx_a/bsu_N. IPR020003. ATPase_F1/V1/A1_a/bsu_AS. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Pfam	PF00006. ATP-synt_ab. 2 hits. PF00306. ATP-synt_ab_C. 1 hit. PF02874. ATP-synt_ab_N. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS00152. ATPASE_ALPHA_BETA. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VATB_METKA

Accession

Primary (citable) accession number: Q8TUT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2002
Last modified:	November 24, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)