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Q8TUS2

- RTCB_METKA

UniProt

Q8TUS2 - RTCB_METKA

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Protein
tRNA-splicing ligase RtcB
Gene
rtcB, MK1682
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs Inferred.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi583 – 5831Zinc Reviewed prediction
Metal bindingi700 – 7001Zinc Reviewed prediction
Metal bindingi732 – 7321Zinc Reviewed prediction

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. RNA ligase activity Source: UniProtKB
  3. endonuclease activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
  3. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Ligase, Nuclease

Keywords - Biological processi

Intron homing, tRNA processing

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing ligase RtcB (EC:6.5.1.-)
Cleaved into the following chain:
Gene namesi
Name:rtcB
Ordered Locus Names:MK1682
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100tRNA-splicing ligase RtcB, 1st part Reviewed prediction
PRO_0000232526Add
BLAST
Chaini101 – 582482Mka hyp2 intein Reviewed prediction
PRO_0000232527Add
BLAST
Chaini583 – 988406tRNA-splicing ligase RtcB, 2nd part Reviewed prediction
PRO_0000232528Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi190192.MK1682.

Structurei

3D structure databases

ProteinModelPortaliQ8TUS2.
SMRiQ8TUS2. Positions 7-101, 582-988.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini251 – 415165DOD-type homing endonuclease
Add
BLAST

Sequence similaritiesi

Belongs to the RtcB family.

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000296282.
KOiK14415.
OMAiVHNCGVR.

Family and domain databases

Gene3Di2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR001233. RtcB.
[Graphical view]
PANTHERiPTHR11118. PTHR11118. 1 hit.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF01139. RtcB. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF103365. SSF103365. 2 hits.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS01288. UPF0027. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TUS2-1 [UniParc]FASTAAdd to Basket

« Hide

MAPKSMLKHI RNNVVWELPE DYKGCMKVPG RIYATEKLID GMEKGVFDQV    50
ANVACLPGIY GYSIALPDAH YGYGFPIGGV AAFDVEEGVV SPGGVGYDIN 100
CLAPGTKILT EHGCWVKVED LPKMLTDQKL KVYDVDEGRE DDSEIKFVME 150
RGIEEDERAV VLVTESGLTI EGSEDHPVLT PEGYVELGEI EEGDLVVVYP 200
FEGVEYEEKE GTILDESDFE DVDPQVLRYL EERDLIPLRW SDPKVGTLAR 250
ILGFAMGDGH LGEQAGRLTL SFYGDERTLR ELKRDLESLG VKANLHVRKR 300
RYEIETASGR YEGEATSVEL RVASRSFALL MEKLGMPRGR KVETPYKVPD 350
WIKEAPLWVK RNFLAGLFAA DGSVVKFKRY TPLPINLTQA KVEELEENLR 400
EFMNDVAKLL REFGIETTLY EVKSKKNVVY KLAIVGEENI KRFLGKVGYE 450
YDPEKKVEGL AAYAYLKLKE RVKKDRKEAA ETAAEVYEET GSITKAHEAV 500
ADVVNRRFVE RVVYDGGISS VRVPEDFPTF ERFKEERVLA GGFVIEEVVE 550
VKGVEPEYDR FYDIGVCHGA HNFIADGVVV HNCGVRVMKT DLTEDDVRPK 600
LRELLETIFR NVPAGLGSRH RRVRLSTQEL RQVMLYGAEW AVEEGFGFDE 650
DLDHIESRGN MTHAYETIGW EEYGPRDDVA SKRAIERGRP QLGTLGSGNH 700
FLEVQVVDEI YDKEAAEKMG IREEGQVTIM VHTGSRGFGH QVCSDHLRIM 750
ERSMRDVERR FGVRIPDRQL ACAAMGTDEA KRYFNAMNAA ANYAFANRQM 800
ISHWTRESFV EVFGDEYGDA DDMGIEVIYD IAHNMAKIEK HPVDGEERWL 850
VVHRKGATRA FSEEALKKHG EPVPFEGLPQ PVLIPGDMGT GSYILIGTEK 900
AMEETWGSTC HGAGRTMSRA AAKRKFWGED VARELERQGI LVKAASMPVV 950
AEEAPPAYKD VDEVVRAVAE AGISDPVVRL RPIGVVKG 988
Length:988
Mass (Da):110,989
Last modified:June 1, 2002 - v1
Checksum:iC24DAC047EAC3132
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM02895.1.
RefSeqiNP_614965.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM02895; AAM02895; MK1682.
GeneIDi1478277.
KEGGimka:MK1682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM02895.1 .
RefSeqi NP_614965.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TUS2.
SMRi Q8TUS2. Positions 7-101, 582-988.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK1682.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM02895 ; AAM02895 ; MK1682 .
GeneIDi 1478277.
KEGGi mka:MK1682.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000296282.
KOi K14415.
OMAi VHNCGVR.

Family and domain databases

Gene3Di 2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR001233. RtcB.
[Graphical view ]
PANTHERi PTHR11118. PTHR11118. 1 hit.
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF01139. RtcB. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF103365. SSF103365. 2 hits.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS01288. UPF0027. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
  2. "Archaeal 3'-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation."
    Englert M., Sheppard K., Aslanian A., Yates J.R. III, Soll D.
    Proc. Natl. Acad. Sci. U.S.A. 108:1290-1295(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT.
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiRTCB_METKA
AccessioniPrimary (citable) accession number: Q8TUS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi