Reviewed, UniProtKB/Swiss-Prot Q8TUS2 (Y1682_METKA)

Names and origin

Protein names

Recommended name:
    UPF0027 protein MK1682
Cleaved into the following chain:
    1- Recommended name:
            Mka hyp2 intein
              EC=3.1.-.-

Gene names

Ordered Locus Names:

MK1682

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the UPF0027 (rtcB) family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 100	100	UPF0027 protein MK1682, 1st part Potential		PRO_0000232526
	Chain	101 – 582	482	Mka hyp2 intein Potential		PRO_0000232527
	Chain	583 – 988	406	UPF0027 protein MK1682, 2nd part Potential		PRO_0000232528
Regions
	Domain	251 – 415	165	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q8TUS2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C24DAC047EAC3132	FASTA	988	110,989
	10 20 30 40 50 60 MAPKSMLKHI RNNVVWELPE DYKGCMKVPG RIYATEKLID GMEKGVFDQV ANVACLPGIY 70 80 90 100 110 120 GYSIALPDAH YGYGFPIGGV AAFDVEEGVV SPGGVGYDIN CLAPGTKILT EHGCWVKVED 130 140 150 160 170 180 LPKMLTDQKL KVYDVDEGRE DDSEIKFVME RGIEEDERAV VLVTESGLTI EGSEDHPVLT 190 200 210 220 230 240 PEGYVELGEI EEGDLVVVYP FEGVEYEEKE GTILDESDFE DVDPQVLRYL EERDLIPLRW 250 260 270 280 290 300 SDPKVGTLAR ILGFAMGDGH LGEQAGRLTL SFYGDERTLR ELKRDLESLG VKANLHVRKR 310 320 330 340 350 360 RYEIETASGR YEGEATSVEL RVASRSFALL MEKLGMPRGR KVETPYKVPD WIKEAPLWVK 370 380 390 400 410 420 RNFLAGLFAA DGSVVKFKRY TPLPINLTQA KVEELEENLR EFMNDVAKLL REFGIETTLY 430 440 450 460 470 480 EVKSKKNVVY KLAIVGEENI KRFLGKVGYE YDPEKKVEGL AAYAYLKLKE RVKKDRKEAA 490 500 510 520 530 540 ETAAEVYEET GSITKAHEAV ADVVNRRFVE RVVYDGGISS VRVPEDFPTF ERFKEERVLA 550 560 570 580 590 600 GGFVIEEVVE VKGVEPEYDR FYDIGVCHGA HNFIADGVVV HNCGVRVMKT DLTEDDVRPK 610 620 630 640 650 660 LRELLETIFR NVPAGLGSRH RRVRLSTQEL RQVMLYGAEW AVEEGFGFDE DLDHIESRGN 670 680 690 700 710 720 MTHAYETIGW EEYGPRDDVA SKRAIERGRP QLGTLGSGNH FLEVQVVDEI YDKEAAEKMG 730 740 750 760 770 780 IREEGQVTIM VHTGSRGFGH QVCSDHLRIM ERSMRDVERR FGVRIPDRQL ACAAMGTDEA 790 800 810 820 830 840 KRYFNAMNAA ANYAFANRQM ISHWTRESFV EVFGDEYGDA DDMGIEVIYD IAHNMAKIEK 850 860 870 880 890 900 HPVDGEERWL VVHRKGATRA FSEEALKKHG EPVPFEGLPQ PVLIPGDMGT GSYILIGTEK 910 920 930 940 950 960 AMEETWGSTC HGAGRTMSRA AAKRKFWGED VARELERQGI LVKAASMPVV AEEAPPAYKD 970 980 VDEVVRAVAE AGISDPVVRL RPIGVVKG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009439 Genomic DNA. Translation: AAM02895.1.
RefSeq	NP_614965.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1478277.
GenomeReviews	Gene locus MK1682 in contig AE009439_GR.
KEGG	mka:MK1682.
NMPDR	fig|190192.1.peg.1678.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TUS2.
OMA	VHNCGVR
Enzyme and pathway databases
BioCyc	MKAN190192:MK1682-MON.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR001233. UPF0027. [Graphical view]
PANTHER	PTHR11118. UPF0027. 1 hit.
Pfam	PF01139. UPF0027. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS01288. UPF0027. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1682_METKA

Accession

Primary (citable) accession number: Q8TUS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)