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Q8TUS2 (RTCB_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA-splicing ligase RtcB

EC=6.5.1.-

Cleaved into the following chain:

  1. Mka hyp2 intein
    EC=3.1.-.-
Gene names
Name:rtcB
Ordered Locus Names:MK1682
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length988 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs Probable. Ref.2

Subunit structure

Monomer. Ref.2

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the RtcB family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 100100tRNA-splicing ligase RtcB, 1st part Potential
PRO_0000232526
Chain101 – 582482Mka hyp2 intein Potential
PRO_0000232527
Chain583 – 988406tRNA-splicing ligase RtcB, 2nd part Potential
PRO_0000232528

Regions

Domain251 – 415165DOD-type homing endonuclease

Sites

Metal binding5831Zinc Potential
Metal binding7001Zinc Potential
Metal binding7321Zinc Potential

Sequences

Sequence LengthMass (Da)Tools
Q8TUS2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C24DAC047EAC3132

FASTA988110,989
        10         20         30         40         50         60 
MAPKSMLKHI RNNVVWELPE DYKGCMKVPG RIYATEKLID GMEKGVFDQV ANVACLPGIY 

        70         80         90        100        110        120 
GYSIALPDAH YGYGFPIGGV AAFDVEEGVV SPGGVGYDIN CLAPGTKILT EHGCWVKVED 

       130        140        150        160        170        180 
LPKMLTDQKL KVYDVDEGRE DDSEIKFVME RGIEEDERAV VLVTESGLTI EGSEDHPVLT 

       190        200        210        220        230        240 
PEGYVELGEI EEGDLVVVYP FEGVEYEEKE GTILDESDFE DVDPQVLRYL EERDLIPLRW 

       250        260        270        280        290        300 
SDPKVGTLAR ILGFAMGDGH LGEQAGRLTL SFYGDERTLR ELKRDLESLG VKANLHVRKR 

       310        320        330        340        350        360 
RYEIETASGR YEGEATSVEL RVASRSFALL MEKLGMPRGR KVETPYKVPD WIKEAPLWVK 

       370        380        390        400        410        420 
RNFLAGLFAA DGSVVKFKRY TPLPINLTQA KVEELEENLR EFMNDVAKLL REFGIETTLY 

       430        440        450        460        470        480 
EVKSKKNVVY KLAIVGEENI KRFLGKVGYE YDPEKKVEGL AAYAYLKLKE RVKKDRKEAA 

       490        500        510        520        530        540 
ETAAEVYEET GSITKAHEAV ADVVNRRFVE RVVYDGGISS VRVPEDFPTF ERFKEERVLA 

       550        560        570        580        590        600 
GGFVIEEVVE VKGVEPEYDR FYDIGVCHGA HNFIADGVVV HNCGVRVMKT DLTEDDVRPK 

       610        620        630        640        650        660 
LRELLETIFR NVPAGLGSRH RRVRLSTQEL RQVMLYGAEW AVEEGFGFDE DLDHIESRGN 

       670        680        690        700        710        720 
MTHAYETIGW EEYGPRDDVA SKRAIERGRP QLGTLGSGNH FLEVQVVDEI YDKEAAEKMG 

       730        740        750        760        770        780 
IREEGQVTIM VHTGSRGFGH QVCSDHLRIM ERSMRDVERR FGVRIPDRQL ACAAMGTDEA 

       790        800        810        820        830        840 
KRYFNAMNAA ANYAFANRQM ISHWTRESFV EVFGDEYGDA DDMGIEVIYD IAHNMAKIEK 

       850        860        870        880        890        900 
HPVDGEERWL VVHRKGATRA FSEEALKKHG EPVPFEGLPQ PVLIPGDMGT GSYILIGTEK 

       910        920        930        940        950        960 
AMEETWGSTC HGAGRTMSRA AAKRKFWGED VARELERQGI LVKAASMPVV AEEAPPAYKD 

       970        980 
VDEVVRAVAE AGISDPVVRL RPIGVVKG 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[2]"Archaeal 3'-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation."
Englert M., Sheppard K., Aslanian A., Yates J.R. III, Soll D.
Proc. Natl. Acad. Sci. U.S.A. 108:1290-1295(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT.
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM02895.1.
RefSeqNP_614965.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TUS2.
SMRQ8TUS2. Positions 7-101, 582-988.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK1682.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM02895; AAM02895; MK1682.
GeneID1478277.
KEGGmka:MK1682.

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000296282.
KOK14415.
OMAVHNCGVR.

Family and domain databases

Gene3D2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
InterProIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR001233. RtcB.
[Graphical view]
PANTHERPTHR11118. PTHR11118. 1 hit.
PfamPF14528. LAGLIDADG_3. 1 hit.
PF01139. RtcB. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF103365. SSF103365. 2 hits.
SSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS01288. UPF0027. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCB_METKA
AccessionPrimary (citable) accession number: Q8TUS2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries