Reviewed, UniProtKB/Swiss-Prot Q8TUQ0 (CHEB2_METAC)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	MA_0015

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000158052
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	161 – 356	196	CheB-type methylesterase
Sites
	Active site	173	1	By similarity
	Active site	200	1	By similarity
	Active site	300	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TUQ0-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 64442F9927EFF8CC	FASTA	356	38,594
	10 20 30 40 50 60 MTIRALVVDD SALIRKVLSD ILNEDPKIRV VGTAVNGKDG LEKVIKLRPD VVLLDNVMPV 70 80 90 100 110 120 LDGLKTLARI MKEQPTPVVI VSALGERAEE ITLTAFEYGA VDVIEKPSGI LSQSMPEMAE 130 140 150 160 170 180 EICRKVRTAP KANLKNLECM RDSEPLNPEK RETKENRVLK KAAPRNILAI GASTGGPRAL 190 200 210 220 230 240 EKLICSLPAE LPAAVLVVQH MPPGFTASLS KRLDSKSALR VKEAQEGDRV EDGTVLIAPG 250 260 270 280 290 300 NYHMEIVRNK VNSLEEETIH LSCGPKELGS RPSVNVLFRS IASVYGSRVI SLVLTGMNCD 310 320 330 340 350 GADGAEEIKK MGGKVIAEAR SSCVVYGMPG EIVRRNLADL VLPLDKMAEE IIRIIG

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM03469.1.
RefSeq	NP_614989.1.
3D structure databases
HSSP	HSSP built from PDB template 1TMY based on UniProtKB Q56312.
ModBase	Search...
Genome annotation databases
GeneID	1471907.
GenomeReviews	Gene locus MA_0015 in contig AE010299_GR.
KEGG	mac:MA0015.
NMPDR	fig|188937.1.peg.15.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TUQ0.
OMA	NCDGADG.
Enzyme and pathway databases
BioCyc	MACE188937:MA0015-MON.
BRENDA	3.1.1.61. 275708.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_METAC

Accession

Primary (citable) accession number: Q8TUQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)