Q8TUD7 (PUR5_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	MA_0130

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_A.
Sequence similarities	Belongs to the AIR synthase family.
Sequence caution	The sequence AAM03584.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A		PRO_0000148280

Sequences

	Sequence		Length	Mass (Da)
	Q8TUD7 [UniParc]. Last modified November 28, 2003. Version 2. Checksum: E097F24B54A097CD	FASTA	333	36,098
	10 20 30 40 50 60 MSEKHLTYAD SGVDITKEEK TVKTLIDKLS YVRKGIGAPL TGIGHYAGLL DFGEYALAMT 70 80 90 100 110 120 TDGVGSKVLI ANEMKRWNTV GIDCIAMNVN DLLAIGAEPV AFVDYLALEK HEEGFAAQIG 130 140 150 160 170 180 EGLLKGAEIS RMSIVGGETA TLPDIIKGFD LAGTCLGVVK KEDILEGGKV RVGDVLVGIP 190 200 210 220 230 240 STGVHSNGYT LVRKIIEKSG YSYHDPCPYD SSKTIGDELL EPTRIYIEVL DVLKACEVHG 250 260 270 280 290 300 LAHITGSGLL KLRRVTKLGF EFSDPIEPQE IFKFLQKEGE VEDLEMYRTF NMGMGFLMIL 310 320 330 PEKDAAKAAE LTGGKIVGKI VESGIRVKDL IIE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03584.1. Different initiation.
RefSeq	NP_615104.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TUD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472022.
GenomeReviews	Gene locus MA_0130 in contig AE010299_GR.
KEGG	mac:MA0130.
NMPDR	fig|188937.1.peg.130.
Phylogenomic databases
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MACE188937:MA0130-MONOMER.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_METAC

Accession

Primary (citable) accession number: Q8TUD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program