Q8TU04 (MTRF_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit F
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F

Gene names

Name:	mtrF
Ordered Locus Names:	MA_0271

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	74 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01099
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01099
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01099
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential HAMAP MF_01099.
Sequence similarities	Belongs to the mtrF family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 74	74	Tetrahydromethanopterin S-methyltransferase subunit F HAMAP MF_01099		PRO_0000147546
Regions
	Transmembrane	51 – 71	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TU04 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B59D7617AA57ECCA	FASTA	74	7,989
	10 20 30 40 50 60 MRMAEEYGKG VPMVLNPQMG AIDATVESIR YRAQLIARNQ KLDSGVMSTG IIGFAAGFLF 70 SLLMVIILPL MAGL

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03724.1.
RefSeq	NP_615244.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472163.
GenomeReviews	Gene locus MA_0271 in contig AE010299_GR.
KEGG	mac:MA0271.
NMPDR	fig|188937.1.peg.270.
Phylogenomic databases
HOGENOM	HBG542040.
OMA	KLESGIM.
ProtClustDB	PRK13275.
Enzyme and pathway databases
BioCyc	MACE188937:MA0271-MONOMER.
Family and domain databases
HAMAP	MF_01099. MtrF. [Tree]
InterPro	IPR011307. MeTrfase_F. IPR013347. MeTrfase_F_su. [Graphical view]
KO	K00582.
Pfam	PF09472. MtrF. 1 hit. [Graphical view]
PIRSF	PIRSF006523. MtrF. 1 hit.
TIGRFAMs	TIGR02507. MtrF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRF_METAC

Accession

Primary (citable) accession number: Q8TU04

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program