Reviewed, UniProtKB/Swiss-Prot Q8TU01 (MTRC_METAC)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit C
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit C

Gene names

Name:	mtrC
Ordered Locus Names:	MA_0274

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01096
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01096
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the mtrC family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Tetrahydromethanopterin S-methyltransferase subunit C HAMAP MF_01096		PRO_0000147520
Regions
	Transmembrane	19 – 39	21	Potential
	Transmembrane	40 – 60	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	96 – 116	21	Potential
	Transmembrane	131 – 151	21	Potential
	Transmembrane	162 – 182	21	Potential
	Transmembrane	221 – 241	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TU01-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: FD258F13BE4D92ED	FASTA	267	26,950
	10 20 30 40 50 60 MSAGGAGGEA KGGFPPQTIM AIGAIGGLAG IYLGNFMPAQ FSFFGGLGAI CAMVWGADAV 70 80 90 100 110 120 RRVASYGLGT GVPSIGMISL GMGIVAALFG LSVGGIAGPI VSFIAAAIIG AVIGVLANKV 130 140 150 160 170 180 IGMGIPIMEQ AMVEIAGAGT LVIIGLSVVI AGTFDYAEVV EYVVANGYIA LIFIIGGMGI 190 200 210 220 230 240 LHPFNANLGP DEKQDRTLSV AVEKAAIALI ITGFASSLHE GLMAAGLNIA VGVIIWAWAF 250 260 MKYYGYVKRD SYAVVGTGLL PSAEELE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM03727.1.
RefSeq	NP_615247.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1472166.
GenomeReviews	Gene locus MA_0274 in contig AE010299_GR.
KEGG	mac:MA0274.
NMPDR	fig|188937.1.peg.273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TU01.
OMA	Q8TU01. ILHPFNA.
Enzyme and pathway databases
BioCyc	MACE188937:MA0274-MON.
BRENDA	2.1.1.86. 275708.
Family and domain databases
HAMAP	MF_01096. [Tree]
InterPro	IPR005865. THM_MeTrfase_su_C. [Graphical view]
Pfam	PF04211. MtrC. 1 hit. [Graphical view]
PIRSF	PIRSF006530. MtrC. 1 hit.
TIGRFAMs	TIGR01148. mtrC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRC_METAC

Accession

Primary (citable) accession number: Q8TU01

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)