Q8TU00 (MTRD_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit D
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D

Gene names

Name:	mtrD
Ordered Locus Names:	MA_0275

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01097
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01097
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01097
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential HAMAP MF_01097.
Sequence similarities	Belongs to the mtrD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Tetrahydromethanopterin S-methyltransferase subunit D HAMAP MF_01097		PRO_0000147528
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TU00 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 85A9E4CAA18B808D	FASTA	249	25,352
	10 20 30 40 50 60 MIDALMANIL WLVFIIIGGV LISWSVHFVP VGGAPAAMAQ ATGIGTGTVQ LAAGAGLTGL 70 80 90 100 110 120 VSAGFMMNVT DNLPLILASG AVGAMIMISV TMIVGTWVYV YGVGCVPSSA KVKYDPITKY 130 140 150 160 170 180 RQDLYVSQGT EGHGLPTVSF VSGVIGGLLG GIGGALVYYS LIEVGLTAGL STGTSSGVTG 190 200 210 220 230 240 HELVGIAAMF AIGIFFVNAV IPSYNIGGTI EGFHDPKWKK WPKAVISSFV ATILCAIVAV IAISQLGGI

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03728.1.
RefSeq	NP_615248.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472167.
GenomeReviews	Gene locus MA_0275 in contig AE010299_GR.
KEGG	mac:MA0275.
NMPDR	fig|188937.1.peg.274.
Phylogenomic databases
HOGENOM	HBG551221.
OMA	VHFVPVG.
ProtClustDB	PRK00968.
Enzyme and pathway databases
BioCyc	MACE188937:MA0275-MONOMER.
Family and domain databases
HAMAP	MF_01097. MtrD. [Tree]
InterPro	IPR005779. MeTrfase_D. [Graphical view]
KO	K00580.
Pfam	PF04207. MtrD. 1 hit. [Graphical view]
PIRSF	PIRSF016552. MtrD. 1 hit.
ProDom	PD013594. MeTrfase_D. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01112. MtrD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRD_METAC

Accession

Primary (citable) accession number: Q8TU00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program