Q8TTZ9 (MTRE_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit E
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E

Gene names

Name:	mtrE
Ordered Locus Names:	MA_0276

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01098
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01098
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01098
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential HAMAP MF_01098.
Sequence similarities	Belongs to the mtrE family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Tetrahydromethanopterin S-methyltransferase subunit E HAMAP MF_01098		PRO_0000147536
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	131 – 151	21	Helical; Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	263 – 283	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TTZ9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C5BEF98875C5FCAC	FASTA	304	32,434
	10 20 30 40 50 60 MEPLIGMGVL ALIGVAATIA GASEDLESDV GSQSNPNSQV QLAPQMMFPH RIFNKAVSGE 70 80 90 100 110 120 PPSNALMCSV GAAVATVLIS EFTLSPLFAL VLGALIAACV HGTFAVTSTM GRAASQSRFK 130 140 150 160 170 180 QPVYLDMIRS HTPVIMGYSF ITTFCILVVS YLMTVVLGHP FPLTMLAFIW GITVGAIGSS 190 200 210 220 230 240 TGDVHYGAER EFQQFEFGSG LNASNSGNIV RYGESGVRNG YDNSWFCAKF GGPVTGMAFG 250 260 270 280 290 300 MTVFLGSWVT TVFDPAVSIS RGWISVVAGV IIVLILIFWN WKIEVKARNA YGPYKEDKTE EASA

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03729.1.
RefSeq	NP_615249.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472168.
GenomeReviews	Gene locus MA_0276 in contig AE010299_GR.
KEGG	mac:MA0276.
NMPDR	fig|188937.1.peg.275.
Phylogenomic databases
HOGENOM	HBG539646.
OMA	SNPNSQV.
ProtClustDB	PRK00972.
Enzyme and pathway databases
BioCyc	MACE188937:MA0276-MONOMER.
Family and domain databases
HAMAP	MF_01098. MtrE. [Tree]
InterPro	IPR005780. MeTrfase_E. [Graphical view]
KO	K00581.
Pfam	PF04206. MtrE. 1 hit. [Graphical view]
PIRSF	PIRSF016509. MtrE. 1 hit.
TIGRFAMs	TIGR01113. MtrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRE_METAC

Accession

Primary (citable) accession number: Q8TTZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program