Reviewed, UniProtKB/Swiss-Prot Q8TTA3 (SYK1_METAC)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase 1
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase 1
      Short name=LysRS 1

Gene names

Name:	lysS1
Synonyms:	lysK
Ordered Locus Names:	MA_0534

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00177
Subcellular location	Cytoplasm. HAMAP MF_00177
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Lysyl-tRNA synthetase 1 HAMAP MF_00177		PRO_0000152751
Regions
	Motif	26 – 34	9	"HIGH" region HAMAP MF_00177
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00177

Sequences

	Sequence		Length	Mass (Da)
	Q8TTA3-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 718C638082F601B6	FASTA	533	59,593
	10 20 30 40 50 60 MHWADVIAED VLNKSGKHLV ATGITPSGHI HIGNMREVVT ADAAYRALLD MGADARLIYI 70 80 90 100 110 120 ADNYDPLRKV YPFLPESYAE HVGKPISEVP CPCGDCANYA EHFLKPFIEA LRRLGINPEV 130 140 150 160 170 180 LRADEMYRAG LYTEAIKTAL AKRDEIAKIL EEVSGKTVAE DWSPFNPRCN ECGKITTTKV 190 200 210 220 230 240 AGFDLEAETV DYVCACGHSG TVPMAGGGKL TWRVDWPARW AVLGVTVEPF GKDHASKGGS 250 260 270 280 290 300 YDTGKRIVRE IYGHEPPFPI VYEWIMLGKR GAMSSSTGVV VSISDMLEVV PPEVLRYLII 310 320 330 340 350 360 RTKPEKHIQF DPGQPLLNLV DEYERLRDKF RENDPSLGDF EKRIYELSRA TGICHPEIPF 370 380 390 400 410 420 KQMVTIYQVA RGDFEQVLKI VKRSGFSTEN EKCIRELADN VSRWLELYAP PFAKFSVKEK 430 440 450 460 470 480 VPVQTATLSE LQKAFLGAFA DLIETKGKIS GEEYHMLVYS AKDEGSELNR LIAQKVNVPV 490 500 510 520 530 PQVDPKDLFK AIYTSILGQS SGPKAGWFLS SFEKGFLITR FREASTYNPE KSS

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM03978.1.
RefSeq	NP_615498.1.
3D structure databases
HSSP	HSSP built from PDB template 1IRX based on UniProtKB O57963.
ModBase	Search...
Genome annotation databases
GeneID	1472426.
GenomeReviews	Gene locus MA_0534 in contig AE010299_GR.
KEGG	mac:MA0534.
NMPDR	fig|188937.1.peg.524.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TTA3.
OMA	TTITHFD.
Enzyme and pathway databases
BioCyc	MACE188937:MA0534-MON.
BRENDA	6.1.1.6. 275708.
Family and domain databases
HAMAP	MF_00177. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR002904. Lys-tRNA-synth_Ic_arc-type. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
Pfam	PF01921. tRNA-synt_1f. 1 hit. [Graphical view]
TIGRFAMs	TIGR00467. lysS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK1_METAC

Accession

Primary (citable) accession number: Q8TTA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)