Reviewed, UniProtKB/Swiss-Prot Q8TT94 (PIMT2_METAC)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate O-methyltransferase 2
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase 2
      Short name=PIMT 2
    Protein L-isoaspartyl methyltransferase 2
    L-isoaspartyl protein carboxyl methyltransferase 2

Gene names

Name:	pcm2
Synonyms:	pcm-2
Ordered Locus Names:	MA_0543

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: HAMAP protein repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Protein-L-isoaspartate O-methyltransferase 2 HAMAP MF_00090		PRO_0000111915
Sites
	Active site	82	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TT94-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9B7EF47C884E035F	FASTA	238	26,970
	10 20 30 40 50 60 MVLVNAISEK DYIKDSRRRK KEEKEFEKLR KLLVEGLEDI VRDKKVLKAM FRVPRHRFVP 70 80 90 100 110 120 EYEQRAAYTD RPLEIGHGQT ISTPHTVALM CEILELSEGH KVLEIGTGSG YNAAVMAELV 130 140 150 160 170 180 GKTGHIYSVE RIEPLVNFAR KNLEQMGYDN VTVLLENGSM GYPRYAPYDR IAVTCAAPTI 190 200 210 220 230 PKALLEQLKP GGIMVIPVGD YSQELIRVKK DSNGNIYKKR KGEVIFVPML GRHGFPRS

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM03987.1.
RefSeq	NP_615507.1.
3D structure databases
HSSP	HSSP built from PDB template 1JG1 based on UniProtKB Q8TZR3.
ModBase	Search...
Genome annotation databases
GeneID	1472435.
GenomeReviews	Gene locus MA_0543 in contig AE010299_GR.
KEGG	mac:MA0543.
NMPDR	fig|188937.1.peg.533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TT94.
OMA	Q8TT94. IFVEEAM.
Enzyme and pathway databases
BioCyc	MACE188937:MA0543-MON.
BRENDA	2.1.1.77. 275708.
Family and domain databases
HAMAP	MF_00090. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT2_METAC

Accession

Primary (citable) accession number: Q8TT94

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)