Q8TT89 (RIBB_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	MA_0548

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Subunit structure	Homodimer By similarity. HAMAP MF_00180
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_0000151824
Regions
	Region	38 – 39	2	Substrate binding By similarity
	Region	179 – 183	5	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese 1 By similarity
	Metal binding	39	1	Magnesium or manganese 2 By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Site	165	1	Essential for catalytic activity By similarity
	Site	203	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TT89 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 214C3716BFDD17EE	FASTA	247	27,396
	10 20 30 40 50 60 MNESTVYECL KYGNENINRA LEVLRAGKMI QIYDSDSREG ETDLVIPAKA VTYKDVKWMR 70 80 90 100 110 120 KDAGGLICVA VDPVASKQLK LPFMADLVRE ASRTSESLGE VVEKDGDLKY DSHSSFSIWV 130 140 150 160 170 180 NHRDTRTGIP DLERALTIRK IGEITEKSLS GNGIRFGNEF RTPGHVALLR AAEGLLDERM 190 200 210 220 230 240 GQTELSVALA RMAGITPAMV VCEMLDDESG RALSKENSKD YGKDHGLVFL EGQEILEAYM LWTGSEC

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03992.1.
RefSeq	NP_615512.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TT89.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472440.
GenomeReviews	Gene locus MA_0548 in contig AE010299_GR.
KEGG	mac:MA0548.
NMPDR	fig|188937.1.peg.538.
Phylogenomic databases
HOGENOM	HBG735778.
OMA	GDMIFAA.
ProtClustDB	CLSK634377.
Enzyme and pathway databases
BioCyc	MACE188937:MA0548-MONOMER.
Family and domain databases
HAMAP	MF_00180. RibB. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K02858.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00506. RibB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_METAC

Accession

Primary (citable) accession number: Q8TT89

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program