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Q8TT52 (SYE_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MA_0587
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAM04031.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119716

Regions

Motif110 – 12011"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q8TT52 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 58D020CFF44934EF

FASTA57164,566
        10         20         30         40         50         60 
MTLSPEDLKT IEKYALQNAV KYEKAPQSKA VMGKVMGECP QLRANPGAVS TALKSIVSEI 

        70         80         90        100        110        120 
AKGNPEAWKT RLSEIAPELI EALNVKKEPE KGLKPLEGAE PGKVVMRFAP NPNGPGTLGS 

       130        140        150        160        170        180 
ARGMVVNSEY VKMYKGKFVL RFDDTDPDIK RPMLEAYDWY LDDFKWLGVV PDRVVYASDH 

       190        200        210        220        230        240 
FPIYYDYARK LIEMGKAYVC FCKGEDFKRL KDAKQACPHR DTSPEENLMH WEKMLAGEYE 

       250        260        270        280        290        300 
DQQAVLRIKT DIEHKDPALR DWGAFRIRKM SHPRAEIGNK YVVWPLLDFA GAIEDHELGM 

       310        320        330        340        350        360 
THIIRGKDLI DSEKRQGYIY KYFGWTYPKT THWGRVKIHE FGKFSTSSLR KAIEAGEYSG 

       370        380        390        400        410        420 
WDDPRLPTIR AIRRRGIQAE ALKKFMIEMG VGMTDVSISM ESLYAENRKI VDPVANRYFF 

       430        440        450        460        470        480 
VWAPVELEIA GMEPTVAKLP LHPTDHSRGV REIAVGNKVL VCAEDVEKLE LGSVIRLKDF 

       490        500        510        520        530        540 
CNIEITSLSP LQAKHSDVSL EALKKAKAKI VHWAPVDGIS VKVRGPEGDL EGIGEKGIVG 

       550        560        570 
ELDKIVQFER FGFCRIDAVS EEKVVAYFAH K 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM04031.1. Different initiation.
RefSeqNP_615551.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TT52.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA0587.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM04031; AAM04031; MA_0587.
GeneID1472479.
KEGGmac:MA0587.

Phylogenomic databases

eggNOGCOG0008.
KOK01885.
OMAAFRIIDT.
PhylomeDBQ8TT52.

Enzyme and pathway databases

BioCycMACE188937:GI2O-593-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_METAC
AccessionPrimary (citable) accession number: Q8TT52
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries