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Q8TT52

- SYE_METAC

UniProt

Q8TT52 - SYE_METAC

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMACE188937:GI2O-593-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:MA_0587
    OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
    Taxonomic identifieri188937 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000002487: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 571571Glutamate--tRNA ligasePRO_0000119716Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi188937.MA0587.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TT52.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi110 – 12011"HIGH" regionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01885.
    OMAiAFRIIDT.
    PhylomeDBiQ8TT52.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    2.40.240.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_A. Glu_tRNA_synth_A.
    InterProiIPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR022888. Glu_tRNA_synth_arc.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8TT52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLSPEDLKT IEKYALQNAV KYEKAPQSKA VMGKVMGECP QLRANPGAVS    50
    TALKSIVSEI AKGNPEAWKT RLSEIAPELI EALNVKKEPE KGLKPLEGAE 100
    PGKVVMRFAP NPNGPGTLGS ARGMVVNSEY VKMYKGKFVL RFDDTDPDIK 150
    RPMLEAYDWY LDDFKWLGVV PDRVVYASDH FPIYYDYARK LIEMGKAYVC 200
    FCKGEDFKRL KDAKQACPHR DTSPEENLMH WEKMLAGEYE DQQAVLRIKT 250
    DIEHKDPALR DWGAFRIRKM SHPRAEIGNK YVVWPLLDFA GAIEDHELGM 300
    THIIRGKDLI DSEKRQGYIY KYFGWTYPKT THWGRVKIHE FGKFSTSSLR 350
    KAIEAGEYSG WDDPRLPTIR AIRRRGIQAE ALKKFMIEMG VGMTDVSISM 400
    ESLYAENRKI VDPVANRYFF VWAPVELEIA GMEPTVAKLP LHPTDHSRGV 450
    REIAVGNKVL VCAEDVEKLE LGSVIRLKDF CNIEITSLSP LQAKHSDVSL 500
    EALKKAKAKI VHWAPVDGIS VKVRGPEGDL EGIGEKGIVG ELDKIVQFER 550
    FGFCRIDAVS EEKVVAYFAH K 571
    Length:571
    Mass (Da):64,566
    Last modified:April 30, 2003 - v2
    Checksum:i58D020CFF44934EF
    GO

    Sequence cautioni

    The sequence AAM04031.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04031.1. Different initiation.
    RefSeqiNP_615551.1. NC_003552.1.

    Genome annotation databases

    EnsemblBacteriaiAAM04031; AAM04031; MA_0587.
    GeneIDi1472479.
    KEGGimac:MA0587.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04031.1 . Different initiation.
    RefSeqi NP_615551.1. NC_003552.1.

    3D structure databases

    ProteinModelPortali Q8TT52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 188937.MA0587.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM04031 ; AAM04031 ; MA_0587 .
    GeneIDi 1472479.
    KEGGi mac:MA0587.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01885.
    OMAi AFRIIDT.
    PhylomeDBi Q8TT52.

    Enzyme and pathway databases

    BioCyci MACE188937:GI2O-593-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    2.40.240.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_A. Glu_tRNA_synth_A.
    InterProi IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR022888. Glu_tRNA_synth_arc.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
      Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
      , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
      Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

    Entry informationi

    Entry nameiSYE_METAC
    AccessioniPrimary (citable) accession number: Q8TT52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3