Q8TSV8 (HDRE_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CoB--CoM heterodisulfide reductase 2 subunit E
EC=1.8.98.1

Gene names

Name:	hdrE
Ordered Locus Names:	MA_0687

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrE may be responsible for anchoring the complex to the membrane By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 2 is composed of two subunits; hdrD and hdrE By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the hdrE family.

Ontologies

Keywords
Biological process	Methanogenesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC nitrate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	CoB--CoM heterodisulfide reductase 2 subunit E		PRO_0000150082
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	185 – 205	21	Helical; Potential
	Transmembrane	223 – 243	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TSV8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: EC385BA2219A5FC8	FASTA	264	29,527
	10 20 30 40 50 60 MSSEMAYFSG LTDALRLTFV QIMILSTIAI VVFLYGMILN FQKWGAGVTG YALEPQAGSK 70 80 90 100 110 120 GSAIRFLKTW WGQVVEESHH GHGKPILEVL ILDILFQRRI LKRSPLRWFM HFTIFAGWMT 130 140 150 160 170 180 LFALSGLMFA VEMTEKFGIE LPFTPAEFRE FLSIPNYIFG YILLIGVLIA LVRRIVVSDV 190 200 210 220 230 240 REASIMYDWI LIGGVFLVTI SGFVADGIRT GIIWGFGLDP TTAPPAALFH SVISLFFCIA 250 260 YIPYSKYIHV IATPLAILAN KGGE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04127.1.
RefSeq	NP_615647.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TSV8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472579.
GenomeReviews	Gene locus MA_0687 in contig AE010299_GR.
KEGG	mac:MA0687.
NMPDR	fig|188937.1.peg.673.
Phylogenomic databases
HOGENOM	HBG538650.
OMA	LANKGGE.
ProtClustDB	CLSK568762.
Enzyme and pathway databases
BioCyc	MACE188937:MA0687-MONOMER.
Family and domain databases
InterPro	IPR023234. NarG-like_domain. IPR003816. Nitrate_red_gam. [Graphical view]
KO	K08265.
Pfam	PF02665. Nitrate_red_gam. 1 hit. [Graphical view]
SUPFAM	SSF103501. NarG-like_domain. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDRE_METAC

Accession

Primary (citable) accession number: Q8TSV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program