Reviewed, UniProtKB/Swiss-Prot Q8TSV7 (HDRD_METAC)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase 2 iron-sulfur subunit D
    EC=1.8.98.1

Gene names

Name:	hdrD
Ordered Locus Names:	MA_0688

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrD may act as the catalytic subunit By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 2 is composed of two subunits; hdrD and hdrE By similarity.
Sequence similarities	Belongs to the hdrD family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	CoB--CoM heterodisulfide reductase 2 iron-sulfur subunit D		PRO_0000150079
Regions
	Domain	14 – 44	31	4Fe-4S ferredoxin-type 1
	Domain	81 – 110	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	24	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	27	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	30	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	34	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	90	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	96	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	100	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TSV7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 579201A5293BC83F	FASTA	409	45,202
	10 20 30 40 50 60 MAKKTPSIDT KNLTAVQLME LDSCTRCGEC VKWCPTYAAS GQKPGLAPRD KILRWRQYMN 70 80 90 100 110 120 KSYGLKAKLF GPTEIPQSEL EEFKDDVHGC TTCGICATVC ESGINTVELW ESLRTNLVKK 130 140 150 160 170 180 GIGPFGKQGM FPKLIGQYHN PYLLDQKDRL AWVPPDVKIA DKANIVYFTG CTAGYKQLAL 190 200 210 220 230 240 AFATSRVLNK LGIEFTMLGE DEWCCGSALI RTGQVHVNDV AKELAKHNVE AIKAKGATKV 250 260 270 280 290 300 LYACAGCFRA SKVDWPRLLG EELPFEVVHI TEFLEDLIKK DKIKWEKSLD KTVTYHDPCH 310 320 330 340 350 360 LGRHVGVFEP PRYVLSHIPG VKFVEMDRVK EFQRCCGAGG GVKAGIPDLA LSVAESRVKD 370 380 390 400 ALDTNADVLS SACPFCKRNL MDGRDSLKAD IEVEDVIVLV AQALGLSVE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM04128.1.
RefSeq	NP_615648.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1472580.
GenomeReviews	Gene locus MA_0688 in contig AE010299_GR.
KEGG	mac:MA0688.
NMPDR	fig|188937.1.peg.674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TSV7.
OMA	Q8TSV7. QRCCGAG.
Enzyme and pathway databases
BioCyc	MACE188937:MA0688-MON.
BRENDA	1.8.98.1. 275708.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR004017. Cys_rich_region. IPR012285. Fum_reductase_C. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF02754. CCG. 2 hits. [Graphical view]
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRD_METAC

Accession

Primary (citable) accession number: Q8TSV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)