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Q8TSS8 (HPRT_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine/guanine phosphoribosyltransferase

Short name=HGPRTase
EC=2.4.2.8
Gene names
Name:hpt
Ordered Locus Names:MA_0717
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis By similarity. HAMAP-Rule MF_01467

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01467

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01467

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1. HAMAP-Rule MF_01467

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01467

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01467.

Disruption phenotype

Cells have increased resistant to purine analogs 8-aza-2,6-diaminopurine (8-ADP) and 2-methylpurine (2MP). No change in growth rate in HS (minimal) broth. Ref.2

Biotechnological use

Used for negative selection when transforming this organism. Ref.2

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processIMP salvage

Inferred from electronic annotation. Source: UniProtKB-UniPathway

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionguanine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

hypoxanthine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Hypoxanthine/guanine phosphoribosyltransferase HAMAP-Rule MF_01467
PRO_0000415480

Sequences

Sequence LengthMass (Da)Tools
Q8TSS8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 3378267CF6A6B848

FASTA18920,673
        10         20         30         40         50         60 
MLERLKDSLV NSPVIKRGEY NYFIHPISDG VPSIDPRLIE EIANYIIRIA DMDVDTILTI 

        70         80         90        100        110        120 
EAMGIPVANA LSLKTGIPLT IVRKRPYFLE GEVELSQSTG YSKGVLYING LKKGDRVVIV 

       130        140        150        160        170        180 
DDVISTGGTL LALVKALQNM GVEITDVISV IGRGAGYFKL RELGVEPKIL VTIDVSEKGV 


EIQDVFGDQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM04157.1.
RefSeqNP_615677.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TSS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA0717.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM04157; AAM04157; MA_0717.
GeneID1472609.
KEGGmac:MA0717.

Phylogenomic databases

eggNOGCOG0503.
KOK00759.
OMANIDSEYF.
PhylomeDBQ8TSS8.

Enzyme and pathway databases

BioCycMACE188937:GI2O-728-MONOMER.
UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
HAMAPMF_01467. Hypx_phosphoribosyltr.
InterProIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_METAC
AccessionPrimary (citable) accession number: Q8TSS8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways