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Q8TSS8

- HPRT_METAC

UniProt

Q8TSS8 - HPRT_METAC

Protein

Hypoxanthine/guanine phosphoribosyltransferase

Gene

hpt

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMACE188937:GI2O-728-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRTase
    Gene namesi
    Name:hpt
    Ordered Locus Names:MA_0717
    OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
    Taxonomic identifieri188937 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000002487: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Biotechnological usei

    Used for negative selection when transforming this organism.1 Publication

    Disruption phenotypei

    Cells have increased resistant to purine analogs 8-aza-2,6-diaminopurine (8-ADP) and 2-methylpurine (2MP). No change in growth rate in HS (minimal) broth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 189189Hypoxanthine/guanine phosphoribosyltransferasePRO_0000415480Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi188937.MA0717.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TSS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0503.
    KOiK00759.
    OMAiNIDSEYF.
    PhylomeDBiQ8TSS8.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_01467. Hypx_phosphoribosyltr.
    InterProiIPR026597. HGPRTase-like.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TSS8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLERLKDSLV NSPVIKRGEY NYFIHPISDG VPSIDPRLIE EIANYIIRIA    50
    DMDVDTILTI EAMGIPVANA LSLKTGIPLT IVRKRPYFLE GEVELSQSTG 100
    YSKGVLYING LKKGDRVVIV DDVISTGGTL LALVKALQNM GVEITDVISV 150
    IGRGAGYFKL RELGVEPKIL VTIDVSEKGV EIQDVFGDQ 189
    Length:189
    Mass (Da):20,673
    Last modified:June 1, 2002 - v1
    Checksum:i3378267CF6A6B848
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04157.1.
    RefSeqiNP_615677.1. NC_003552.1.

    Genome annotation databases

    EnsemblBacteriaiAAM04157; AAM04157; MA_0717.
    GeneIDi1472609.
    KEGGimac:MA0717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04157.1 .
    RefSeqi NP_615677.1. NC_003552.1.

    3D structure databases

    ProteinModelPortali Q8TSS8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 188937.MA0717.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM04157 ; AAM04157 ; MA_0717 .
    GeneIDi 1472609.
    KEGGi mac:MA0717.

    Phylogenomic databases

    eggNOGi COG0503.
    KOi K00759.
    OMAi NIDSEYF.
    PhylomeDBi Q8TSS8.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci MACE188937:GI2O-728-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    HAMAPi MF_01467. Hypx_phosphoribosyltr.
    InterProi IPR026597. HGPRTase-like.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
      Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
      , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
      Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.
    2. "Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea."
      Pritchett M.A., Zhang J.K., Metcalf W.W.
      Appl. Environ. Microbiol. 70:1425-1433(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY, DISRUPTION PHENOTYPE.
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

    Entry informationi

    Entry nameiHPRT_METAC
    AccessioniPrimary (citable) accession number: Q8TSS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3