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Protein

Hypoxanthine/guanine phosphoribosyltransferase

Gene

hpt

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. hypoxanthine salvage Source: UniProtKB-HAMAP
  2. IMP salvage Source: UniProtKB-UniPathway
  3. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMACE188937:GI2O-728-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine/guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRTase
Gene namesi
Name:hpt
Ordered Locus Names:MA_0717
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Used for negative selection when transforming this organism.1 Publication

Disruption phenotypei

Cells have increased resistant to purine analogs 8-aza-2,6-diaminopurine (8-ADP) and 2-methylpurine (2MP). No change in growth rate in HS (minimal) broth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Hypoxanthine/guanine phosphoribosyltransferasePRO_0000415480Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi188937.MA0717.

Structurei

3D structure databases

ProteinModelPortaliQ8TSS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0503.
InParanoidiQ8TSS8.
KOiK00759.
OMAiKIICAVE.
PhylomeDBiQ8TSS8.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TSS8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLERLKDSLV NSPVIKRGEY NYFIHPISDG VPSIDPRLIE EIANYIIRIA
60 70 80 90 100
DMDVDTILTI EAMGIPVANA LSLKTGIPLT IVRKRPYFLE GEVELSQSTG
110 120 130 140 150
YSKGVLYING LKKGDRVVIV DDVISTGGTL LALVKALQNM GVEITDVISV
160 170 180
IGRGAGYFKL RELGVEPKIL VTIDVSEKGV EIQDVFGDQ
Length:189
Mass (Da):20,673
Last modified:June 1, 2002 - v1
Checksum:i3378267CF6A6B848
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM04157.1.
RefSeqiNP_615677.1. NC_003552.1.
WP_011020762.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM04157; AAM04157; MA_0717.
GeneIDi1472609.
KEGGimac:MA0717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM04157.1.
RefSeqiNP_615677.1. NC_003552.1.
WP_011020762.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TSS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA0717.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM04157; AAM04157; MA_0717.
GeneIDi1472609.
KEGGimac:MA0717.

Phylogenomic databases

eggNOGiCOG0503.
InParanoidiQ8TSS8.
KOiK00759.
OMAiKIICAVE.
PhylomeDBiQ8TSS8.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciMACE188937:GI2O-728-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01467. Hypx_phosphoribosyltr.
InterProiIPR026597. HGPRTase-like.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.
  2. "Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea."
    Pritchett M.A., Zhang J.K., Metcalf W.W.
    Appl. Environ. Microbiol. 70:1425-1433(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY, DISRUPTION PHENOTYPE.
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiHPRT_METAC
AccessioniPrimary (citable) accession number: Q8TSS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.