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Protein

DNA ligase 1

Gene

lig1

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei245 – 2451ATPUniRule annotation
Active sitei247 – 2471N6-AMP-lysine intermediateUniRule annotation
Binding sitei252 – 2521ATPUniRule annotation
Binding sitei267 – 2671ATPUniRule annotation
Binding sitei297 – 2971ATPUniRule annotation
Binding sitei343 – 3431ATPUniRule annotation
Binding sitei420 – 4201ATPUniRule annotation
Binding sitei426 – 4261ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: GO_Central
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation Source: GO_Central
  5. DNA ligation involved in DNA repair Source: InterPro
  6. DNA recombination Source: UniProtKB-HAMAP
  7. lagging strand elongation Source: GO_Central
  8. nucleotide-excision repair Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:GI2O-739-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1UniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1UniRule annotation
Gene namesi
Name:lig1UniRule annotation
Ordered Locus Names:MA_0728
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580DNA ligase 1PRO_0000365257Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi188937.MA0728.

Structurei

3D structure databases

ProteinModelPortaliQ8TSR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
InParanoidiQ8TSR7.
KOiK10747.
OMAiASIDIGC.
PhylomeDBiQ8TSR7.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TSR7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRFKELAEL FEELERITSH KEIVRKLAGF FGGLKGDEVK DSAYLFLGSI
60 70 80 90 100
GPAFENTTLG IKDRLVTRAI AGAYGVSEEE VKKRYARAGD LGDVAFELSK
110 120 130 140 150
KREASLTIEE VFKRLRQIKE ASGKGSQEKK TGLLSDILQK ATPEEGKYII
160 170 180 190 200
RIVLGRLRLG FGDQFLLEAF SIAFTGDKKH AGKIKESYSV CTDIGELAQT
210 220 230 240 250
LAEHGAGAPG YFSIKPGRPV KSMLAQRVES FEELEERIKG KKAAEEKYDG
260 270 280 290 300
ERVQIHKAGD EIKAFSRRLE DITAQYPDII EAVRESISAD TIVLDGEIVA
310 320 330 340 350
YAELEKENTR IEEFYPFQNL MQRRRKYEVE KYREKCPVAV FFFDILYLNG
360 370 380 390 400
ESLLKKTYPE RRALLEEHVN GSGIVHLTRR TVTENIEELE DFFNETVEKG
410 420 430 440 450
LEGIVAKSMS SNSVYEAGKR SWLWLKWKQE YSEGMRETFD LVVVGSYYGK
460 470 480 490 500
GKRKGSFGAL LCAVLNEEEQ QFETFTKVGT GFTEADAEEI NSLLSAHTVS
510 520 530 540 550
EAPKNVIIKK GMLPDIFIEP AVVIEVLGSE ITNSPGHTAG EGEEETGLAL
560 570 580
RFPRFLRIRY DRAPFDATTV REIRDLKEGI
Length:580
Mass (Da):65,351
Last modified:June 1, 2002 - v1
Checksum:i31C511F878C79E73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM04168.1.
RefSeqiNP_615688.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM04168; AAM04168; MA_0728.
GeneIDi1472620.
KEGGimac:MA0728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM04168.1.
RefSeqiNP_615688.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TSR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA0728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM04168; AAM04168; MA_0728.
GeneIDi1472620.
KEGGimac:MA0728.

Phylogenomic databases

eggNOGiCOG1793.
InParanoidiQ8TSR7.
KOiK10747.
OMAiASIDIGC.
PhylomeDBiQ8TSR7.

Enzyme and pathway databases

BioCyciMACE188937:GI2O-739-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiDNLI1_METAC
AccessioniPrimary (citable) accession number: Q8TSR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.