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Q8TSR7 (DNLI1_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 1

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1
Gene names
Name:lig1
Ordered Locus Names:MA_0728
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580DNA ligase 1 HAMAP-Rule MF_00407
PRO_0000365257

Sites

Active site2471N6-AMP-lysine intermediate By similarity
Binding site2451ATP By similarity
Binding site2521ATP By similarity
Binding site2671ATP By similarity
Binding site2971ATP By similarity
Binding site3431ATP By similarity
Binding site4201ATP By similarity
Binding site4261ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TSR7 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 31C511F878C79E73

FASTA58065,351
        10         20         30         40         50         60 
MVRFKELAEL FEELERITSH KEIVRKLAGF FGGLKGDEVK DSAYLFLGSI GPAFENTTLG 

        70         80         90        100        110        120 
IKDRLVTRAI AGAYGVSEEE VKKRYARAGD LGDVAFELSK KREASLTIEE VFKRLRQIKE 

       130        140        150        160        170        180 
ASGKGSQEKK TGLLSDILQK ATPEEGKYII RIVLGRLRLG FGDQFLLEAF SIAFTGDKKH 

       190        200        210        220        230        240 
AGKIKESYSV CTDIGELAQT LAEHGAGAPG YFSIKPGRPV KSMLAQRVES FEELEERIKG 

       250        260        270        280        290        300 
KKAAEEKYDG ERVQIHKAGD EIKAFSRRLE DITAQYPDII EAVRESISAD TIVLDGEIVA 

       310        320        330        340        350        360 
YAELEKENTR IEEFYPFQNL MQRRRKYEVE KYREKCPVAV FFFDILYLNG ESLLKKTYPE 

       370        380        390        400        410        420 
RRALLEEHVN GSGIVHLTRR TVTENIEELE DFFNETVEKG LEGIVAKSMS SNSVYEAGKR 

       430        440        450        460        470        480 
SWLWLKWKQE YSEGMRETFD LVVVGSYYGK GKRKGSFGAL LCAVLNEEEQ QFETFTKVGT 

       490        500        510        520        530        540 
GFTEADAEEI NSLLSAHTVS EAPKNVIIKK GMLPDIFIEP AVVIEVLGSE ITNSPGHTAG 

       550        560        570        580 
EGEEETGLAL RFPRFLRIRY DRAPFDATTV REIRDLKEGI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM04168.1.
RefSeqNP_615688.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TSR7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA0728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM04168; AAM04168; MA_0728.
GeneID1472620.
KEGGmac:MA0728.

Phylogenomic databases

eggNOGCOG1793.
KOK10747.
OMACTDIGEL.
PhylomeDBQ8TSR7.

Enzyme and pathway databases

BioCycMACE188937:GI2O-739-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI1_METAC
AccessionPrimary (citable) accession number: Q8TSR7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families