Q8TSN5 (SYK2_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase 2
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase 2
Short name=LysRS 2

Gene names

Name:	lysS2
Ordered Locus Names:	MA_0760

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subunit structure	Homodimer By similarity. HAMAP MF_00252
Subcellular location	Cytoplasm HAMAP MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lysine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Lysine--tRNA ligase 2 HAMAP MF_00252		PRO_0000152711
Sites
	Metal binding	421	1	Magnesium 1 By similarity
	Metal binding	428	1	Magnesium 1 By similarity
	Metal binding	428	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TSN5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2478F0DA2479926F	FASTA	511	58,811
	10 20 30 40 50 60 MTMEINNTDP SENMPLPDDV DLSGSRAFDD SKLAKLNGII SQGLDPYPYR FEKNGDICEI 70 80 90 100 110 120 LVKFEDFEKN EGLSVRTAGR LYNIRKHGKM IFADLGDQTG RIQVLIRKGN LPDEEFATFK 130 140 150 160 170 180 NLMDSGDIIG IQGELFRTKR GENSISVSEF SLLSKSLCAL PEKFHGLKDV ETRYRKRYLD 190 200 210 220 230 240 LIVNAEKREI FVMRSKLISE IRRFLADREF LEFETPILQT VYGGANARPF KTFHNCLGQN 250 260 270 280 290 300 LFLRIAPELY LKRLVVGGYE KVFEISKNFR NEDIDTTHNP EFTMIEVYEA YRDYNDMMDL 310 320 330 340 350 360 TEALISELVF RLTGGYEVKM GENTINLRSP WKRISMEGAL KEYAGLDVFS HSLEELKQIA 370 380 390 400 410 420 IQNRIEDYEK AKSHGEFLAL LFEGLVEDKL VNPTFIYDFP VENSPLAKNH REKEGFVERF 430 440 450 460 470 480 ELFLNGWELA NGYSELNDPL EQEKRFEEQD KKRKLGDLEA QTVDYDFINA LGYGLPPTGG 490 500 510 MGLGIDRLTM ILAGLESIKE VILFPQMKRE D

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04200.1.
RefSeq	NP_615720.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TSN5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472652.
GenomeReviews	Gene locus MA_0760 in contig AE010299_GR.
KEGG	mac:MA0760.
NMPDR	fig|188937.1.peg.746.
Phylogenomic databases
HOGENOM	HBG631383.
OMA	MEINNTD.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	MACE188937:MA0760-MONOMER.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K04567.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. LysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK2_METAC

Accession

Primary (citable) accession number: Q8TSN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program