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Q8TSH7

- MDH_METAC

UniProt

Q8TSH7 - MDH_METAC

Protein

Malate dehydrogenase

Gene

mdh

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321NADUniRule annotation
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei87 – 871SubstrateUniRule annotation
    Binding sitei94 – 941NADUniRule annotation
    Binding sitei119 – 1191SubstrateUniRule annotation
    Binding sitei150 – 1501SubstrateUniRule annotation
    Active sitei174 – 1741Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 136NADUniRule annotation
    Nucleotide bindingi117 – 1193NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMACE188937:GI2O-831-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:MA_0819
    OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
    Taxonomic identifieri188937 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000002487: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307Malate dehydrogenasePRO_0000113484Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi188937.MA0819.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TSH7.
    SMRiQ8TSH7. Positions 3-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    KOiK00024.
    OMAiYAPSAFV.
    PhylomeDBiQ8TSH7.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8TSH7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKISVIGAG NVGATTVQRL AELEPGEIVM TDIVEGLPQG KALDLMQAGA    50
    INGYDTQVTG TNDYADITDS DLVIITAGIA RKPGMTREDL MKTNSKIIGE 100
    VSRNIAEYAP NSIVINVTNP LDVITYVAMK TTGFETKKVF GMSGVLDAGR 150
    FASFIAEELN CSKKDIEAMV IGGHGDLMVP LPQYTTVSGI PLPELLPEET 200
    IARLVERTVN GGAEIVGLLK QGSAFYAPSA AIVSVAEAVL KDSKRILPTS 250
    AYLEGQYGQE GIYFGVLAKL GANGVEEVLE LKLEENQYEI LRKSSETIKR 300
    GISKLGI 307
    Length:307
    Mass (Da):32,698
    Last modified:June 1, 2002 - v1
    Checksum:i2A3B07763C9CEBCC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04258.1.
    RefSeqiNP_615778.1. NC_003552.1.
    WP_011020863.1. NC_003552.1.

    Genome annotation databases

    EnsemblBacteriaiAAM04258; AAM04258; MA_0819.
    GeneIDi1472711.
    KEGGimac:MA0819.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM04258.1 .
    RefSeqi NP_615778.1. NC_003552.1.
    WP_011020863.1. NC_003552.1.

    3D structure databases

    ProteinModelPortali Q8TSH7.
    SMRi Q8TSH7. Positions 3-304.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 188937.MA0819.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM04258 ; AAM04258 ; MA_0819 .
    GeneIDi 1472711.
    KEGGi mac:MA0819.

    Phylogenomic databases

    eggNOGi COG0039.
    KOi K00024.
    OMAi YAPSAFV.
    PhylomeDBi Q8TSH7.

    Enzyme and pathway databases

    BioCyci MACE188937:GI2O-831-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
      Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
      , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
      Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

    Entry informationi

    Entry nameiMDH_METAC
    AccessioniPrimary (citable) accession number: Q8TSH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3