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Q8TSH7 (MDH_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:MA_0819
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113484

Regions

Nucleotide binding8 – 136NAD By similarity
Nucleotide binding117 – 1193NAD By similarity

Sites

Active site1741Proton acceptor By similarity
Binding site321NAD By similarity
Binding site811Substrate By similarity
Binding site871Substrate By similarity
Binding site941NAD By similarity
Binding site1191Substrate By similarity
Binding site1501Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TSH7 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2A3B07763C9CEBCC

FASTA30732,698
        10         20         30         40         50         60 
MAKISVIGAG NVGATTVQRL AELEPGEIVM TDIVEGLPQG KALDLMQAGA INGYDTQVTG 

        70         80         90        100        110        120 
TNDYADITDS DLVIITAGIA RKPGMTREDL MKTNSKIIGE VSRNIAEYAP NSIVINVTNP 

       130        140        150        160        170        180 
LDVITYVAMK TTGFETKKVF GMSGVLDAGR FASFIAEELN CSKKDIEAMV IGGHGDLMVP 

       190        200        210        220        230        240 
LPQYTTVSGI PLPELLPEET IARLVERTVN GGAEIVGLLK QGSAFYAPSA AIVSVAEAVL 

       250        260        270        280        290        300 
KDSKRILPTS AYLEGQYGQE GIYFGVLAKL GANGVEEVLE LKLEENQYEI LRKSSETIKR 


GISKLGI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM04258.1.
RefSeqNP_615778.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TSH7.
SMRQ8TSH7. Positions 3-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA0819.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM04258; AAM04258; MA_0819.
GeneID1472711.
KEGGmac:MA0819.

Phylogenomic databases

eggNOGCOG0039.
KOK00024.
OMAGANSYEA.
PhylomeDBQ8TSH7.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycMACE188937:GI2O-831-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_METAC
AccessionPrimary (citable) accession number: Q8TSH7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families