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Q8TS47 (ASPD_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable L-aspartate dehydrogenase

EC=1.4.1.21
Gene names
Name:nadX
Ordered Locus Names:MA_0958
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP-Rule MF_01265

Catalytic activity

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP-Rule MF_01265

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP-Rule MF_01265

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.

Sequence similarities

Belongs to the L-aspartate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Probable L-aspartate dehydrogenase HAMAP-Rule MF_01265
PRO_0000144895

Sites

Active site2221 By similarity
Binding site1241NAD; via amide nitrogen By similarity
Binding site1921NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TS47 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 06D17290784BBBDA

FASTA27128,578
        10         20         30         40         50         60 
MLKIGIVGCG FIGGQICRAI DGGEVSAELY ALCDSSESKA LELAASLKTC KPSYMKIEEL 

        70         80         90        100        110        120 
IRGVDLIIES ASQNAVRFIV PQALKAGCDV MILSVGALAD EELRDTLFGL AKEHNCKLYF 

       130        140        150        160        170        180 
PSGAVVGIDG LNSASAAGIS SVTLSTRKPP AGLMGAPYVV EHGIELEKLE KKTVLFEGPA 

       190        200        210        220        230        240 
SEAVKAFPAN VNVAATISLA GIGFERTRVK VIADPSLFRN VHEIIVEGEF GKFSTRVENL 

       250        260        270 
PSPENPKTSY LAALSAVSTL KKILNPVQIG T 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM04391.1.
RefSeqNP_615911.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TS47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA0958.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM04391; AAM04391; MA_0958.
GeneID1472848.
KEGGmac:MA0958.

Phylogenomic databases

eggNOGCOG1712.
KOK06989.
OMAPKTSYLA.
PhylomeDBQ8TS47.
ProtClustDBPRK13304.

Enzyme and pathway databases

BioCycMACE188937:GI2O-969-MONOMER.
UniPathwayUPA00253; UER00456.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01265. NadX.
InterProIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR011182. Asp_DH_NAD_syn.
IPR020626. Asp_DH_NAD_syn_prok.
IPR022487. Asp_DH_NAD_synth_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsTIGR03855. NAD_NadX. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASPD_METAC
AccessionPrimary (citable) accession number: Q8TS47
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways