Q8TS34 (KATG_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MA_0972

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Catalase-peroxidase HAMAP MF_01961		PRO_0000354968
Sites
	Active site	102	1	Proton acceptor By similarity
	Metal binding	265	1	Iron (heme axial ligand) By similarity
	Site	98	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	101 ↔ 224		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-250) By similarity
	Cross-link	224 ↔ 250		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-101) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TS34 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 67A0508CD91BFA7A	FASTA	736	81,051
	10 20 30 40 50 60 MGGNVMTDDK MNSVTSGANK QETGRDMSNR DWWPNHLKLE ILHQHSSKSN PMGEDFNYAK 70 80 90 100 110 120 EFKSLDLAAV KKDLAALMTD SQDWWPADFG HYGPLFIRMA WHSAGTYRAG DGRGGGGRGQ 130 140 150 160 170 180 QRFAPLNSWP DNVNLDKARR LLWPIKQKYG RKISWADLMI LTGNVAMETM GFKTFGFGGG 190 200 210 220 230 240 REDVWEPDQD VYWGSEDTWL GDERYTGDRD LENPLAAVQM GLIYVNPEGP NGNPDPIAAA 250 260 270 280 290 300 KDIREVFARM AMNDEETVAL IAGGHAFGKT HGAGPASHVG PEPEAASIEA QGLGWKSSFG 310 320 330 340 350 360 TGKGDDTITG GLEVTWTNTP TKWSNNFFRI LFGYEWELTK SPAGAYQWKP KGGAGAGTIP 370 380 390 400 410 420 DAHDPSKRHA PSMMTTDLSL RFDPVYEKIS RHFYENPGQL ADSFARAWFK LTHRDMGPRA 430 440 450 460 470 480 RYLGPEVPAE ELIWQDPIPA VNHELIDEKD IAFLKDRILA SGLSISQLVS TAWVSASTFR 490 500 510 520 530 540 GSDKRGGANG ARIRLAPQKD WEVNQPAELA KVLNTLEGIQ SEFNSAASGG KKVSLADLIV 550 560 570 580 590 600 LAGCAGVEQA AKNAGYDVTV PFLPGRMDAL QEQTDVVSFA LLEPIADGFR NYLKAQYPLP 610 620 630 640 650 660 AEELLVDKAQ LLTLTAPEMT VLVGGMRVLN TNFGHTQHGV FTQKPEALTN DFFVNLLDMG 670 680 690 700 710 720 TEWKAVSDVK DIFEGCDRKT GEVKWTGTRV DLIFGSNSQL RALAEVYGSA DAQEKFVQDF 730 VAAWTKVMNL DRFDLA

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04404.1.
RefSeq	NP_615924.1. NC_003552.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q8TS34.
SMR	Q8TS34. Positions 28-736.
ModBase	Search...
Protein family/group databases
PeroxiBase	2324. MacCP01_C2A.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1472862.
GenomeReviews	Gene locus MA_0972 in contig AE010299_GR.
KEGG	mac:MA0972.
NMPDR	fig|188937.1.peg.950.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	MACE188937:MA0972-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_METAC

Accession

Primary (citable) accession number: Q8TS34

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program