Q8TRK8 (KPRS_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	MA_1167

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00583_A
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00583_A
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP-Rule MF_00583_A
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00583_A.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Ribose-phosphate pyrophosphokinase HAMAP-Rule MF_00583_A		PRO_0000141237
Regions
	Region	201 – 214	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	118	1	Magnesium Potential
	Metal binding	120	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TRK8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 848CB5D1C257237F	FASTA	283	30,384
	10 20 30 40 50 60 MKIIGGPASQ LLASRTARAL GTEPVLCEFN RFPDGELYLR IADEIENEKV TIIQSTPTDS 70 80 90 100 110 120 DFVALLQLID ACEGAAEINV VIPYMGYARQ DKKFKPGESI SARAIARCIN ASRIFTINIH 130 140 150 160 170 180 ERSILDHFPC PAVNLDAASL VGEYIAGSGL ENPMLVAPDE GAQGLVKNVA AGHGFDHDHL 190 200 210 220 230 240 QKTRLSGDTV VIKTKNLDVT GRHVVLVDDM IATGGTMAES VRMLKAQGAI DVHLACVHPV 250 260 270 280 LTRNAALRLF NAGVKDIIAT DTLEKAESLL SVAPLIAEAL KRL

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04588.1.
RefSeq	NP_616108.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TRK8.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA1167.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM04588; AAM04588; MA_1167.
GeneID	1473055.
KEGG	mac:MA1167.
Phylogenomic databases
eggNOG	COG0462.
KO	K00948.
OMA	MQANHPD.
ProtClustDB	PRK00934.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-1179-MONOMER.
UniPathway	UPA00087; UER00172.
Family and domain databases
HAMAP	MF_00583_A. RibP_PPkinase_A.
InterPro	IPR000836. PRibTrfase_dom. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_METAC

Accession

Primary (citable) accession number: Q8TRK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program