Reviewed, UniProtKB/Swiss-Prot Q8TRA5 (SAHH_METAC)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	MA_1275

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000117003
Regions
	Region	173 – 339	167	NAD binding By similarity
Sites
	Binding site	121	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TRA5-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C3221440430631F2	FASTA	411	44,900
	10 20 30 40 50 60 MTEQELVESG NMKMEWARNH MPVIAIIREK FEKEKPLKGL KVGMALHVEA KTAVLVETLA 70 80 90 100 110 120 AGGAQVAISG CNPLSTQDDV ARALDTRKNI SCFARYGCCT SEYYEAIDKV LDIEPDITID 130 140 150 160 170 180 DGADLIFKLH KERTDLLPKI LGGCEETTTG VHRLHAMEKE GALKMPVIAV NDAMTKYLFD 190 200 210 220 230 240 NRYGTGQSAW DGINRTTNLL VAGKNVVVGG YGWCGRGVAM RAAGLGANVI VTEVDPIRAL 250 260 270 280 290 300 EARMDGYRVM RMADAARLGE IFVTTTGNRD ILTAEHFKVM PDGAVLANSG HFNVEIDMEA 310 320 330 340 350 360 LTSLAKSVKT VRHNIKEYDI GDRRINVIAE GRLVNLAAGD GHPAEVMDMS FANQALCVRY 370 380 390 400 410 IAENTLLNGV HGVPRGIDTY VAKLKLESMG ISIDELTSKQ ECYMTGWECG T

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM04694.1.
RefSeq	NP_616214.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI4 based on UniProtKB P23526.
ModBase	Search...
Genome annotation databases
GeneID	1473163.
GenomeReviews	Gene locus MA_1275 in contig AE010299_GR.
KEGG	mac:MA1275.
NMPDR	fig|188937.1.peg.1240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TRA5.
OMA	Q8TRA5. IDDGMDL.
Enzyme and pathway databases
BioCyc	MACE188937:MA1275-MON.
BRENDA	3.3.1.1. 275708.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR000043. Ad_hcy_hydrolase. IPR015878. Ado_hCys_hydrolase_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.1480. Ad_hcy_hydrolase. 1 hit.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_METAC

Accession

Primary (citable) accession number: Q8TRA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)