Carbon monoxide dehydrogenase 2 - Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

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Q8TR73 (COOS2_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Carbon monoxide dehydrogenase 2
Short name=CODH 2
EC=1.2.99.2

Gene names

Name:	cooS2
Ordered Locus Names:	MA_1309

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	CO + H₂O + A = CO₂ + AH₂.
Cofactor	Binds 3 4Fe-4S clusters per homodimer By similarity. Binds 2 nickel-iron-sulfur clusters per homodimer By similarity.
Subunit structure	Homodimer By similarity.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer By similarity.
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family.
Caution	This protein lacks the conserved Cys in positions 52 and 300; they are replaced by an Arg and a Glu, respectively. It is therefore possible that the C- and D-clusters are either altered or missing in this protein.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

633

Carbon monoxide dehydrogenase 2

PRO_0000157142

Sites

Metal binding

1

Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TR73 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 68216C6467807FC1
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633

69,289

        10         20         30         40         50         60 
MDKERISYHE SVQKMYERIK KDNMTNVWDR YEAQGIGGVP DRRCTFCMAG ARCDLCSNGP 

        70         80         90        100        110        120 
CRSDAAKDKR GVCGITADGM AMRMMLLRNV MGASTYHYHT DQTIRTLRET AKGKTPYSIR 

       130        140        150        160        170        180 
EPEKLRTFAG RLGIETLGSD SEIALYLCEF VEKDFNRPAY EPSRIVEILA PPERKKRWEE 

       190        200        210        220        230        240 
LDIFPGGIYG EMMLSTSSCL TNVDGYYASL ALKAMRLGIA MAYQSQIVNE YCQDILFGIP 

       250        260        270        280        290        300 
KPHTMRVDLG VLDPEYVNVL PNGHEPFLGF AMVQLARKPE WQEKAKAAGA KGLRVIASIE 

       310        320        330        340        350        360 
TGQEMIQRWE EDDAFYGFTG NWISQEAVLA SGSVDLFAAD MNCSLPVAPL YAEKYGFKLM 

       370        380        390        400        410        420 
PVSELIAFED ITERLNYNPV EAGRQAAKLL NMAVENFKNR KNSGEPVLNL PVKEAVVGFS 

       430        440        450        460        470        480 
TESILDALGG TLDPLLDAIK SGAIKGVVGM VSCTTLRDYG QDVHSVAVVK ELIKRNILVL 

       490        500        510        520        530        540 
SLGCGNGAMQ VAGLCSPETR EFAGDSLKAV CEALGVPPVL SYGTCTDTGR LADFLGAISA 

       550        560        570        580        590        600 
VMGVPIPDLP IAAAAPEYME QKATIDAIFA LALGLYTYVN PVPTVTGAPD LVKLLTEDCR 

       610        620        630 
EVTGGVLNVE KDAVKAVDGI EQHIMEKRKK LGI

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.

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Birren B.
Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04727.1.
RefSeq	NP_616247.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TR73.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1473197.
GenomeReviews	Gene locus MA_1309 in contig AE010299_GR.
KEGG	mac:MA1309.
NMPDR	fig\|188937.1.peg.1273.
Phylogenomic databases
HOGENOM	HBG393174.
OMA	YMEQKAT.
ProtClustDB	CLSK862738.
Enzyme and pathway databases
BioCyc	MACE188937:MA1309-MONOMER.
Family and domain databases
InterPro	IPR016101. CO_DH_a-bundle. IPR010047. CO_DH_cat. IPR004137. Prismane. IPR011254. Prismane-like. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits.
KO	K00198.
Pfam	PF03063. Prismane. 1 hit. [Graphical view]
PIRSF	PIRSF005023. CODH. 1 hit.
SUPFAM	SSF56821. Prismane_like. 1 hit.
TIGRFAMs	TIGR01702. CO_DH_cata. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COOS2_METAC

Accession

Primary (citable) accession number: Q8TR73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents