Reviewed, UniProtKB/Swiss-Prot Q8TQP1 (FPOH_METAC)

Names and origin

Protein names

Recommended name:
    F(420)H(2) oxidoreductase subunit H
Alternative name(s):
    F(420)H(2) dehydrogenase subunit H
    FPO subunit H

Gene names

Name:	fpoH
Ordered Locus Names:	MA_1499

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Subunit structure	FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	FAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	F(420)H(2) oxidoreductase subunit H HAMAP MF_01350		PRO_0000240121
Regions
	Transmembrane	20 – 40	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	127 – 147	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	198 – 218	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	286 – 306	21	Potential
	Transmembrane	328 – 348	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TQP1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 74B93F24ECAB0A5B	FASTA	348	38,522
	10 20 30 40 50 60 MNIMIEIPEF IIPLIPWIRG VVGLVLVGAI FLGAMGAVWL ERKLSADIQF RYGPSRVGKF 70 80 90 100 110 120 GLLQLVADAI KLFTKEDMRP RNADRLLFDN APIFMMSSVF LMLVAIPVGA VFINGVEYPL 130 140 150 160 170 180 AVTEMDISVL YIEAMSAITI FGIFMIAYGS NNKYSLLGAF RNFARMVGYE VPLGITVVSV 190 200 210 220 230 240 AIMTGSLNIV EIASAQGLLW NIFLQPIGFI VFFIALMADM GRLPFDQNES EEELVAGWIT 250 260 270 280 290 300 EYTGMRFGLG FFAEYIHMIL GSFLVALLFL GGWNVPAFVA NNPVLGLIAP TGFFLLKTVL 310 320 330 340 VLMTIIGMRW AVPRFRIDQV VDLSWKRLLP LSLLNLVWAV GLGLYLGA

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM04913.1.
RefSeq	NP_616433.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1473387.
GenomeReviews	Gene locus MA_1499 in contig AE010299_GR.
KEGG	mac:MA1499.
NMPDR	fig|188937.1.peg.1459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TQP1.
OMA	Q8TQP1. LYLGGWE.
Enzyme and pathway databases
BioCyc	MACE188937:MA1499-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPOH_METAC

Accession

Primary (citable) accession number: Q8TQP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)