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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84NucleophileUniRule annotation1
Binding sitei115L-glutamineUniRule annotation1
Active sitei179Charge relay systemUniRule annotation1
Active sitei181Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-1653-MONOMER
UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:MA_1566
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356791 – 199Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST199

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA1566

Structurei

3D structure databases

ProteinModelPortaliQ8TQH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54L-glutamine bindingUniRule annotation3
Regioni143 – 144L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034 Archaea
COG0311 LUCA
InParanoidiQ8TQH7
KOiK08681
OMAiVFIRAPI
OrthoDBiPOG093Z0AY8
PhylomeDBiQ8TQH7

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TQH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGVIAIQG AVSEHVDALR RALAERGVEA EVVEIKHKGI VPECSGIVIP
60 70 80 90 100
GGESTTLCRL LAREGIGEEI KEAAARGVPV LGTCAGLIVL AKEGDRQVEK
110 120 130 140 150
TGQELLGIMD TRVNRNAFGR QRDSFEAELD VVILDSPFTG VFIRAPGIIS
160 170 180 190
CGPGVRVLSR LEDMIIAAEQ GNVLALAFHP ELTDDLRIHQ YFLNKVLSC
Length:199
Mass (Da):21,392
Last modified:June 1, 2002 - v1
Checksum:iDA8274DCF7BEBE62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM04979.1
RefSeqiWP_011021577.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM04979; AAM04979; MA_1566
GeneIDi1473454
KEGGimac:MA_1566

Similar proteinsi

Entry informationi

Entry nameiPDXT_METAC
AccessioniPrimary (citable) accession number: Q8TQH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: February 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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