Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8TQD3 (SYL_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MA_1611
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length961 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 961961Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152130

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TQD3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8DC8CE4760800A86

FASTA961110,402
        10         20         30         40         50         60 
MEQDYKPHEI EEKWQKKWNE SLIFQADPDK REKFFITIPY PYLNGNLHAG HTRTFTIGDV 

        70         80         90        100        110        120 
VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIASRDPQTM DVYEHLHGIP GDILPTLDTP 

       130        140        150        160        170        180 
EKIVDYFKRE AEKAMRMIGY SIDWRRKFTT TDPTYKKFIE WQYIRLEEKG LIVKGSHPVK 

       190        200        210        220        230        240 
WCPNDNNPVE DHDILYGEEA TIVEYTLIKF RYNDLVLPCA TLRPETTFGV TNLWVNPDVD 

       250        260        270        280        290        300 
YVKARVEKDG NEEFWVVSKE AFRKLTFTDR TVEYVEDVPA KSIIGIKLTN PITGDEVISL 

       310        320        330        340        350        360 
PASFVKPENG SGIVMSVPAH APFDYLALRD LYDADLSEYG ITEDLRDIKL ISLIQVPEFG 

       370        380        390        400        410        420 
EFPAKEIVES MGIANQKAPK AEEATKIVYR REFHGGVLKE ITGKYRGYPV SKIKDVLTRD 

       430        440        450        460        470        480 
LIASNAGETF YEFSEPVVCR CGTPCVVNMV KGQWFLNYSN PEWKAKVYKC LSQMRIIPEE 

       490        500        510        520        530        540 
YRVEFENKVD WLKDKACARR KGLGTRLPFD KEWLIESLGD STIYMSYYII ARFLERGDLA 

       550        560        570        580        590        600 
LEQLTLSFFD YVLLGIGDSA AVSAETGLKQ ELVEEIRSHF NYWYPVDLRS SGKDLVPNHL 

       610        620        630        640        650        660 
LFFLFHHVAL FEEEKWPRAL AVNGFVSLEG QKMSKSKGPI LTLESAVSAY GADITRMYIL 

       670        680        690        700        710        720 
STAEQTQDAD WQKTGIDSAR RQVDRFYSFA KDVIESGKRA TLSTELKLID RWMLSRMQKY 

       730        740        750        760        770        780 
IMETNIALDS IQTREAIQNS FFLLINDVRW YQRRGGEALL YYVLDNWVRL MAPFTPHLCE 

       790        800        810        820        830        840 
EIWEAMGHED PISLAQYPLD NEDLIDEGAE LAEEAVKSTL NDIEEIVRVT KMTPQKVYLY 

       850        860        870        880        890        900 
TAPAWKAEAI RCACELQIEA PLEVGALIKT LMANPELKRF GKEIPKFVQK IIPEFKSGGA 

       910        920        930        940        950        960 
ERYETFAYLG LDEQALLKES ASFLEKEIGC PVEIYSADSP EYDPQKKSRF AEPLRPAIYI 


E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM05024.1.
RefSeqNP_616544.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TQD3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA1611.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM05024; AAM05024; MA_1611.
GeneID1473499.
KEGGmac:MA1611.

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHAPFDYL.
PhylomeDBQ8TQD3.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycMACE188937:GI2O-1627-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METAC
AccessionPrimary (citable) accession number: Q8TQD3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries