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Q8TNY5 (ASSY_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:MA_2142
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148674

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site911Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1721Citrulline By similarity
Binding site1811Citrulline By similarity
Binding site2571Citrulline By similarity
Binding site2691Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TNY5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2B995B9C138E7EF1

FASTA39443,994
        10         20         30         40         50         60 
MVKKVALAYS GGLDTSVCIP ILKEKYGYDE VITISVDVGQ PEEEIKKADA KAEKISNKHY 

        70         80         90        100        110        120 
TIDAKEEFVK DYIFPLIKAN GDYEGYVMGT SVARPLIAKK VVEAAIKEGA VALAHGCTGK 

       130        140        150        160        170        180 
GNDQLRFEAV FRQTDMDVIA PMREMNLTRE WEIDYAKEHG IPVEVTKAKP WSVDENIWSR 

       190        200        210        220        230        240 
SIEGGKLEDP SFVPPEEIFE WTKSAEDAPN EPRIVDIDFE AGVPVALDGE KLGGYALVRK 

       250        260        270        280        290        300 
LNEIAGENGV GRTDMIEDRV LGLKARENYE HPAATVLLAA HADLEKLVLT RGELKFKKIV 

       310        320        330        340        350        360 
DEQWSELAYY GLVDEPLYAD LNAFIDKSQE RVTGTVKVKL YKGALTILAR SSPNALYSED 

       370        380        390 
LVSFDSQTID QKDAEGFAKY HGFQARMYRK VMEK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM05540.1.
RefSeqNP_617060.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TNY5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA2142.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM05540; AAM05540; MA_2142.
GeneID1474030.
KEGGmac:MA2142.

Phylogenomic databases

eggNOGCOG0137.
KOK01940.
OMAAPPEEAY.
PhylomeDBQ8TNY5.

Enzyme and pathway databases

BioCycMACE188937:GI2O-2160-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_METAC
AccessionPrimary (citable) accession number: Q8TNY5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways