Reviewed, UniProtKB/Swiss-Prot Q8TNY3 (CARA_METAC)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	MA_2144

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112357
Regions
	Domain	182 – 368	187	Glutamine amidotransferase type-1
	Region	1 – 175	175	CPSase HAMAP MF_01209
Sites
	Active site	257	1	Nucleophile By similarity
	Active site	342	1	By similarity
	Active site	344	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TNY3-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 3C252F6022D4F06C	FASTA	368	39,829
	10 20 30 40 50 60 MKAVLGLEDG TVIRGTGFGA EGTACGELVF TTQFTGYEEA LTDPSYKGQI LMFTYPLIGN 70 80 90 100 110 120 YGVSGERFQS DNIHAEGLVV REACKKPYHY KSTRSIHQFL EDEGKPGIEG VDTRMLTIGA 130 140 150 160 170 180 RERGTMRAAL ITGSDDGEEA VKVARNFPQI TDEELIARVT CKEPHFIPGA ECAWKGSGKP 190 200 210 220 230 240 KHAVVVDLGI KRNIINNLHK RGIDLTLVPA TTKPKEIAGF EPDLLFISNG PGDPEKATDA 250 260 270 280 290 300 INAVKAFAGT IPVAGICFGH QIISLAMGAR TYKLKFGHRG GNQPVKDLIE NKIFISSQNH 310 320 330 340 350 360 GYAVDADSLE GTGLYVKYLN ANDKTVEGVS HKDLDIFSVQ FHPEAQAGPM DTEETFFGKV VKVLGGDL

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM05542.1.
RefSeq	NP_617062.1.
3D structure databases
HSSP	HSSP built from PDB template 1CS0 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1474032.
GenomeReviews	Gene locus MA_2144 in contig AE010299_GR.
KEGG	mac:MA2144.
NMPDR	fig|188937.1.peg.2088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TNY3.
OMA	Q8TNY3. LFDGSNC.
Enzyme and pathway databases
BioCyc	MACE188937:MA2144-MON.
BRENDA	6.3.5.5. 275708.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_METAC

Accession

Primary (citable) accession number: Q8TNY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)