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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei599ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-2594-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MA_2431
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985831 – 1058Isoleucine--tRNA ligaseAdd BLAST1058

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA2431

Structurei

3D structure databases

ProteinModelPortaliQ8TN62
SMRiQ8TN62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi596 – 600"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
InParanoidiQ8TN62
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054
PhylomeDBiQ8TN62

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TN62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKEITAKYN AEQIEKKVTQ FWEDSDAYRK TRERRKTGKR LFFVDGPPYT
60 70 80 90 100
TGHIHLGTAW NKIIKDTILR YYSMNNRYIL ERPGWDMHGL PIEVRVEGVL
110 120 130 140 150
GFKSKKDIES FGVENFIEKC KEFAITQKQA MTEQFQRLGV WMQWPEPYMT
160 170 180 190 200
LKDDYIEAAW WTLKQAHEKD LLEVGKRSVN WCPRCETAIA DSEVEYSERT
210 220 230 240 250
DPSIYVKFKV KGEENTFIVI WTTTPWTIPA NVAVAVHPAY EYSKFRAIRQ
260 270 280 290 300
DGSEEILIAA TELIKNVLKQ GRYADFKVLE TMLGEELTKL EYESPVGDLV
310 320 330 340 350
PIQNEIKHGV YLADFVTVEN TGCVHIAPGH GMDDFNLGVK HKLPILCPVG
360 370 380 390 400
SNGAYTEEAG EYAGKNVREA NPIVIEDLKA RNRLLAEGTV THRYGHCWRC
410 420 430 440 450
KTPIIYLATE QWFLKITEIK EKMLEEIDAV DWYPDWAGSA RFRTWVEGAR
460 470 480 490 500
DWCISRQRYW GIPLPVWKCK KCGKLEVIGT KAELLEKAGL SGDIELHRPY
510 520 530 540 550
VDRVTVPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT HDQFDEWWPA
560 570 580 590 600
DFVTEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS
610 620 630 640 650
LGNVVSPLDI IDRLGADTLR AYVLSSSAPW EDLKYNLEEV ETVHRSINIL
660 670 680 690 700
WNVFRFPLPY MALDNFDPMQ VSLDSVKDAL REEDRWILSR AQSVIKAVNE
710 720 730 740 750
AMSGYLLHKA VREILEFALE DLSRWYIQLI RPRTWTEADD PDKLAAYCVL
760 770 780 790 800
YEVYVTITKL ISPFMPYLAE EMYQNLIRNV DPNAPESVHM CDWPKVNDTY
810 820 830 840 850
LDPELEVAMD TVRSIVEAAS NARQKAGRKL RWPVSRIIVS PESEAAAKAV
860 870 880 890 900
NRLGSVLMDQ TNSKAIVLTG VGKSWDELGL EVIPDPGKIG PVFKKDAGRV
910 920 930 940 950
IPALQKVEGF TLKKAFAETG EFELTLADGT TVPVTSGMAN FKETLPEGTA
960 970 980 990 1000
SAESDAGLVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIRVS
1010 1020 1030 1040 1050
VRIEAEKVLA LVETLKDLIA EEVRADVFDL GSSIEVSGTL VKDWDVEGTA

MKMGIAKK
Length:1,058
Mass (Da):120,217
Last modified:June 1, 2002 - v1
Checksum:i3940FED68C23AEA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM05817.1
RefSeqiWP_011022402.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM05817; AAM05817; MA_2431
GeneIDi1474320
KEGGimac:MA_2431

Similar proteinsi

Entry informationi

Entry nameiSYI_METAC
AccessioniPrimary (citable) accession number: Q8TN62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health