Q8TMW6 (PNCB_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase
Short name=NAPRTase
EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	MA_2533

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP-Rule MF_00570
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Nicotinate phosphoribosyltransferase HAMAP-Rule MF_00570		PRO_0000205857

Sequences

	Sequence		Length	Mass (Da)
	Q8TMW6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F03CF0523D1A9770	FASTA	404	46,457
	10 20 30 40 50 60 MIKSILDNDL YKFTMQMAVL ELFPKAEAEY RFTNRGSHHF SEEFVEKLRR VIDEDISALM 70 80 90 100 110 120 LTEDEYQWLG ENCSFLKPMY LEYLKNFRFK PGEVEVCLTE EKELDIRIKG PWHSTILWEI 130 140 150 160 170 180 VLMAAVSELY FTTIEKEWNG KEWDGNISAT SESILTAYGE KILEIGKILE ENGCLFAEFG 190 200 210 220 230 240 TRRRRSFELH DQVMKTLLQI ETLTGTSNVF FAKKYGLKPI GTVGHEWIMG TSALIGLRYA 250 260 270 280 290 300 NRFAFENWVE VYKGDLGIAL TDTFGSEAFF KDMDLKLSKI YDGFRHDSGD PFTFVDRVID 310 320 330 340 350 360 HYRKMGIDPM KKVIVFSDAL NAEAAIRLKK YCQDKINCSF GIGTSLTNNS EFFRESPPLN 370 380 390 400 MVIKLHSVNG IPVVKLSDSP EKETGERDAL RVANYIVGRK GLDE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM05916.1.
RefSeq	NP_617436.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TMW6.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA2533.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM05916; AAM05916; MA_2533.
GeneID	1474422.
KEGG	mac:MA2533.
Phylogenomic databases
eggNOG	COG1488.
KO	K00763.
OMA	SFGWGTN.
ProtClustDB	PRK05321.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-2594-MONOMER.
UniPathway	UPA00253; UER00457.
Family and domain databases
HAMAP	MF_00570. NAPRTase.
InterPro	IPR006406. Nic_PRibTrfase. IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01514. NAPRTase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_METAC

Accession

Primary (citable) accession number: Q8TMW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program