Reviewed, UniProtKB/Swiss-Prot Q8TMT1 (DNLI2_METAC)

Names and origin

Protein names

Recommended name:
    DNA ligase 2
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP] 2

Gene names

Name:	lig2
Ordered Locus Names:	MA_2571

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	DNA ligase 2 HAMAP MF_00407		PRO_0000059603
Sites
	Active site	256	1	N6-AMP-lysine intermediate By similarity
	Binding site	254	1	ATP By similarity
	Binding site	261	1	ATP By similarity
	Binding site	276	1	ATP By similarity
	Binding site	306	1	ATP By similarity
	Binding site	346	1	ATP By similarity
	Binding site	425	1	ATP By similarity
	Binding site	431	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TMT1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 64AA8AF2BBAE1261	FASTA	568	63,177
	10 20 30 40 50 60 MTSFREFAET CQAIEKISST IETTNKVADL LKKVDVEELP LATHFIMSEV FPAWSGEQLG 70 80 90 100 110 120 IGTSLLYSSL SKASGMSVRS IESLIRTTGD IGETALLILK EKRKNQVTFS SFLEEQPELS 130 140 150 160 170 180 ITEVYQRFKT ASEASGKGSQ ELKIKNLQFL FNSSTPREAK YISRLALEEL RIGVGEGVVR 190 200 210 220 230 240 DAIARAFSVP SDKVEHAFMV TNDLGIVAAA AKEGGIEALE SLGIEINRPI KMMLSQISPD 250 260 270 280 290 300 IDADIRDMKG AAIEWKFDGA RVQIHKNGNS VTLFSRKLEN VTNSLPDLVE IIRKHVKAES 310 320 330 340 350 360 AILDGEAVAV DENGKPRAFQ EILKRFRRKY DVEEKALGIP IQLNLFDIMY LNGRTLIDLP 370 380 390 400 410 420 LVERRKALES CVESSVEDSK SISVDEQVIT GDLELVEKIY REALNAGHEG VMVKNPNSAY 430 440 450 460 470 480 SPGKRGKNWL KKKPLMETLD LVVVGAEWGY GRRANLIGSY SVACYDPETS RFLQVGKVGT 490 500 510 520 530 540 GLTDEQLKEL TEMLSGLMEG GEAGGVFAIR PKVVLEIAFE EIQKSPNYDS GFALRFPRFI 550 560 RIRDDKDPEE ADTIQRIGRV YSQQLKRL

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM05952.1.
RefSeq	NP_617472.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1474460.
GenomeReviews	Gene locus MA_2571 in contig AE010299_GR.
KEGG	mac:MA2571.
NMPDR	fig|188937.1.peg.2498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TMT1.
OMA	Q8TMT1. SNLHLGA.
Enzyme and pathway databases
BioCyc	MACE188937:MA2571-MON.
BRENDA	6.5.1.1. 275708.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI2_METAC

Accession

Primary (citable) accession number: Q8TMT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)