Reviewed, UniProtKB/Swiss-Prot Q8TMI8 (PGK1_METAC)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase 1
    EC=2.7.2.3

Gene names

Name:	pgk1
Ordered Locus Names:	MA_2669

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Phosphoglycerate kinase 1 HAMAP MF_00145		PRO_0000146054
Regions
	Nucleotide binding	361 – 364	4	ATP By similarity
	Region	28 – 30	3	Substrate binding By similarity
	Region	65 – 68	4	Substrate binding By similarity
Sites
	Binding site	122	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TMI8-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4D41FC977625CEF6	FASTA	412	45,497
	10 20 30 40 50 60 MSQKTAGKDY LTMDDVELDN KRILLRVDFN SPMDANGNIL DDRKIKSHLY TLRSLENSRV 70 80 90 100 110 120 VMMSHQGRPG DKDYTTLEAH AKLATELLGR KVTYEDDIFS ACARNAIKSL EKGDILLLEN 130 140 150 160 170 180 TRFYAEENMN RAPEEQARTQ MVRKLYPLFD VFINDAFSVS HRSQCSVVGF TEVLPSVAGI 190 200 210 220 230 240 LMDREITGLD KGLKCHEHPA VFALGGTKAK DIVKVISDIL KRGGADRILT TGVVATVFMM 250 260 270 280 290 300 AIGIEVGEVN RKFIEDHKYL DQVSIASRLL KEYSGKIIVP KDIALNNDGK REEVKVDKIK 310 320 330 340 350 360 GDLPIADIGP ETISDYSKFL KEAKLSVFHG PAGIFELESF RLGTEELLKA AAQSNYSIAG 370 380 390 400 410 GGHTLAAIDQ LGLESKYSHL SMGGGASITY LSGEHMPGIE ALKNYASRCC KD

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM06047.1.
RefSeq	NP_617567.1.
3D structure databases
HSSP	HSSP built from PDB template 1PHP based on UniProtKB P18912.
ModBase	Search...
Genome annotation databases
GeneID	1474558.
GenomeReviews	Gene locus MA_2669 in contig AE010299_GR.
KEGG	mac:MA2669.
NMPDR	fig|188937.1.peg.2593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TMI8.
OMA	FINDAFS.
Enzyme and pathway databases
BioCyc	MACE188937:MA2669-MON.
BRENDA	2.7.2.3. 275708.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK1_METAC

Accession

Primary (citable) accession number: Q8TMI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)