ID   CAPPA_METAC             Reviewed;         526 AA.
AC   Q8TMG9;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 2.
DT   27-MAR-2024, entry version 97.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904};
GN   OrderedLocusNames=MA_2690;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM06068.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE010299; AAM06068.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011022649.1; NC_003552.1.
DR   AlphaFoldDB; Q8TMG9; -.
DR   SMR; Q8TMG9; -.
DR   STRING; 188937.MA_2690; -.
DR   EnsemblBacteria; AAM06068; AAM06068; MA_2690.
DR   GeneID; 1474582; -.
DR   KEGG; mac:MA_2690; -.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   InParanoid; Q8TMG9; -.
DR   OrthoDB; 85849at2157; -.
DR   PhylomeDB; Q8TMG9; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..526
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309602"
SQ   SEQUENCE   526 AA;  58335 MW;  8FB2AC2F5BED5CFD CRC64;
     MSRKSTYPKV MCTQHPDSAS RYISTQEEPG EAIEAAVVFG CDEYMPDYEG KATPYHQNVQ
     IVSRLIEETD LVPGKDVFIT PRAPSAVQEN RFRQLMVMMS IAEANHGALE YSDVQAINEF
     VHPMTGTVRE ILDAQQHMVD VSELAKKEFG FAMEVPRIIP LIEDAPALLH AKELTENTLL
     AWKERFGTVP EKFRVFLGKS DSALSFGHVA STLSCKYAIN GLSELNFELE TETGIVFGAG
     TLPFRGHLDL KNAENFFREY RGIGTITLQS ALRYSHEKGD AEALVDLAKE KLPEIPELFS
     AEEKEELVNL IGIFGTRYSL IIRELASTIN RLSDLLPQQR DRLMHRGSGG YSRSAPDISG
     IVSLCRTDIG KELLASMPAE DLHLPRAIKF TGALYSIGLP PEFIGTGAAL NEAREKLGDE
     ACERLLTKYF PSLVSDLNFA TGYLDLNVAS RFLSGACFKE VSKDIEILHE TLGLETHPEP
     SYRILLEMMQ PELLQAGTSG NCMDEEVSQL VCSTLTKMGK IRKALG
//