Q8TMG9 (CAPPA_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	MA_2690

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_01904
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_01904
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the PEPCase type 2 family.
Sequence caution	The sequence AAM06068.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904		PRO_0000309602

Sequences

	Sequence		Length	Mass (Da)
	Q8TMG9 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 8FB2AC2F5BED5CFD	FASTA	526	58,335
	10 20 30 40 50 60 MSRKSTYPKV MCTQHPDSAS RYISTQEEPG EAIEAAVVFG CDEYMPDYEG KATPYHQNVQ 70 80 90 100 110 120 IVSRLIEETD LVPGKDVFIT PRAPSAVQEN RFRQLMVMMS IAEANHGALE YSDVQAINEF 130 140 150 160 170 180 VHPMTGTVRE ILDAQQHMVD VSELAKKEFG FAMEVPRIIP LIEDAPALLH AKELTENTLL 190 200 210 220 230 240 AWKERFGTVP EKFRVFLGKS DSALSFGHVA STLSCKYAIN GLSELNFELE TETGIVFGAG 250 260 270 280 290 300 TLPFRGHLDL KNAENFFREY RGIGTITLQS ALRYSHEKGD AEALVDLAKE KLPEIPELFS 310 320 330 340 350 360 AEEKEELVNL IGIFGTRYSL IIRELASTIN RLSDLLPQQR DRLMHRGSGG YSRSAPDISG 370 380 390 400 410 420 IVSLCRTDIG KELLASMPAE DLHLPRAIKF TGALYSIGLP PEFIGTGAAL NEAREKLGDE 430 440 450 460 470 480 ACERLLTKYF PSLVSDLNFA TGYLDLNVAS RFLSGACFKE VSKDIEILHE TLGLETHPEP 490 500 510 520 SYRILLEMMQ PELLQAGTSG NCMDEEVSQL VCSTLTKMGK IRKALG

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06068.1. Different initiation.
RefSeq	NP_617588.2. NC_003552.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA2690.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM06068; AAM06068; MA_2690.
GeneID	1474582.
KEGG	mac:MA2690.
Phylogenomic databases
eggNOG	COG1892.
KO	K01595.
OMA	MCTQHPD.
ProtClustDB	PRK13655.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-2754-MONOMER.
Family and domain databases
HAMAP	MF_01904. PEPcase_type2.
InterPro	IPR007566. PEP_COase_arc-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PIRSF	PIRSF006677. UCP006677. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_METAC

Accession

Primary (citable) accession number: Q8TMG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program