Reviewed, UniProtKB/Swiss-Prot Q8TMD5 (THIM_METAC)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	MA_2723

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156968
Sites
	Metal binding	89	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Binding site	40	1	Substrate; via amide nitrogen By similarity
	Binding site	116	1	ATP By similarity
	Binding site	162	1	ATP By similarity
	Binding site	189	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TMD5-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1C119D21B4CDB056	FASTA	261	27,767
	10 20 30 40 50 60 MNEPLKTIRE TKPLIHHITN WVTIYECANM TRAFGALPVM AHAPEECADM TKISSALVLN 70 80 90 100 110 120 IGTLTSEIID SMLLSAAAAN ERDIPVVLDA VGVGATKFRD EMAAKILASV HIDIIKGNYS 130 140 150 160 170 180 EIAKLAGENA ETKGVEATSI DADPAKVAKA FAKAESCVVV MTGKEDIISD GDRTFIVKNG 190 200 210 220 230 240 HELMGSIVGT GCMAASIVGL FAAVNRDYCD AAKDALCYFG ASGELAAAKS SGPGSFKVHL 250 260 YDEVFNLSDE KVKSMKNFEE K

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM06102.1.
RefSeq	NP_617622.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKQ based on UniProtKB P39593.
ModBase	Search...
Genome annotation databases
GeneID	1474616.
GenomeReviews	Gene locus MA_2723 in contig AE010299_GR.
KEGG	mac:MA2723.
NMPDR	fig|188937.1.peg.2648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TMD5.
OMA	Q8TMD5. ASPVMAH.
Enzyme and pathway databases
BioCyc	MACE188937:MA2723-MON.
BRENDA	2.7.1.50. 275708.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_METAC

Accession

Primary (citable) accession number: Q8TMD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)