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Q8TMD5

- THIM_METAC

UniProt

Q8TMD5 - THIM_METAC

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401Substrate; via amide nitrogenUniRule annotation
Binding sitei116 – 1161ATPUniRule annotation
Binding sitei162 – 1621ATPUniRule annotation
Binding sitei189 – 1891Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:GI2O-2753-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:MA_2723
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Hydroxyethylthiazole kinasePRO_0000156968Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi188937.MA2723.

Structurei

3D structure databases

ProteinModelPortaliQ8TMD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
InParanoidiQ8TMD5.
KOiK00878.
OMAiQTFTANV.
PhylomeDBiQ8TMD5.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TMD5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNEPLKTIRE TKPLIHHITN WVTIYECANM TRAFGALPVM AHAPEECADM
60 70 80 90 100
TKISSALVLN IGTLTSEIID SMLLSAAAAN ERDIPVVLDA VGVGATKFRD
110 120 130 140 150
EMAAKILASV HIDIIKGNYS EIAKLAGENA ETKGVEATSI DADPAKVAKA
160 170 180 190 200
FAKAESCVVV MTGKEDIISD GDRTFIVKNG HELMGSIVGT GCMAASIVGL
210 220 230 240 250
FAAVNRDYCD AAKDALCYFG ASGELAAAKS SGPGSFKVHL YDEVFNLSDE
260
KVKSMKNFEE K
Length:261
Mass (Da):27,767
Last modified:June 1, 2002 - v1
Checksum:i1C119D21B4CDB056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06102.1.
RefSeqiNP_617622.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06102; AAM06102; MA_2723.
GeneIDi1474616.
KEGGimac:MA2723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06102.1 .
RefSeqi NP_617622.1. NC_003552.1.

3D structure databases

ProteinModelPortali Q8TMD5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 188937.MA2723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM06102 ; AAM06102 ; MA_2723 .
GeneIDi 1474616.
KEGGi mac:MA2723.

Phylogenomic databases

eggNOGi COG2145.
InParanoidi Q8TMD5.
KOi K00878.
OMAi QTFTANV.
PhylomeDBi Q8TMD5.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci MACE188937:GI2O-2753-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiTHIM_METAC
AccessioniPrimary (citable) accession number: Q8TMD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3