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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Catalytic activityi

Alpha-D-ribose 1,5-bisphosphate = D-ribulose 1,5-bisphosphate.

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NAD; via amide nitrogenUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi156Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi171IronUniRule annotation1
Binding sitei224NAD; via amide nitrogenUniRule annotation1
Binding sitei234GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi154 – 156NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MA_2851
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539461 – 260Thiamine thiazole synthaseAdd BLAST260

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA2851.

Structurei

3D structure databases

ProteinModelPortaliQ8TM19.
SMRiQ8TM19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
InParanoidiQ8TM19.
KOiK03146.
OMAiMFPRIVV.
OrthoDBiPOG093Z09PU.
PhylomeDBiQ8TM19.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TM19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDEVIITR AIFDEYSKTF LDYTDIDVAL VGGGPANLVA AKYLAEAGVK
60 70 80 90 100
VALYEQKLSL GGGMWAGGMM FPRIVVQEEA TRILDDFGIR YKEYESGYYV
110 120 130 140 150
ANSVESVGKL IAGATSAGAE VFNLVSFEDI MIRENDRVTG IVINWGPVTT
160 170 180 190 200
QRLHVDPLMI RTKLVIDGTG HEAVVCNTIL RKIPNAKIGE LGLLGEKPMW
210 220 230 240 250
SEVGERLAVN ATQEIYPGLI VAGMAANAAT RAPRMGPVFG GMLLSGEKAA
260
KLALDRLKTI
Length:260
Mass (Da):28,097
Last modified:June 1, 2002 - v1
Checksum:i8C2B378141F9E0C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06230.1.
RefSeqiWP_011022803.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06230; AAM06230; MA_2851.
GeneIDi1474748.
KEGGimac:MA_2851.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTHI4_METAC
AccessioniPrimary (citable) accession number: Q8TM19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2002
Last modified: July 5, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

This protein was originally thought to have ribose 1,5-bisphosphate isomerase activity despite the fact that some recombinant ortholog proteins were not active in vitro (PubMed:15375115 and PubMed:17303759). Moreover, another protein from M.acetivorans likely possesses this activity (MA_0379). Finally, a thiamine synthase activity has been shown for the ortholog protein in M.jannaschii.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families