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Protein

Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit A

Gene

hdrA2

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). Catalyzes the transfer of electrons from ferredoxin to CoM-S-S-CoB during methanogenesis from acetate. Electrons transfer from ferredoxin to CoM-S-S-CoB via HdrA2, HdrC2 and HdrB2. In addition, the complex can use electron bifurcation to direct electron pairs from reduced coenzyme F420 towards the reduction of both ferredoxin and CoB-CoM heterodisulfide. This activity may take place during Fe(III)-dependent anaerobic methane oxidation.1 Publication

Catalytic activityi

2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM = 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+.1 Publication
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterPROSITE-ProRule annotation1 PublicationNote: Binds 4 [4Fe-4S] cluster.PROSITE-ProRule annotation1 Publication
  • [2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster.1 Publication
  • FAD1 Publication

Pathwayi: coenzyme M-coenzyme B heterodisulfide reduction

This protein is involved in step 1 of the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydromethanophenazine:CoB--CoM heterodisulfide reductase subunit D (hdrD), Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit B (hdrB2), Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit A (hdrA2), Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit C (hdrC2), Ferredoxin:CoB-CoM heterodisulfide reductase subunit A (hdrA1), Ferredoxin:CoB-CoM heterodisulfide reductase subunit B (hdrB1), Ferredoxin:CoB-CoM heterodisulfide reductase subunit C (hdrC1), Dihydromethanophenazine:CoB--CoM heterodisulfide reductase subunit E (hdrE)
This subpathway is part of the pathway coenzyme M-coenzyme B heterodisulfide reduction, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide, the pathway coenzyme M-coenzyme B heterodisulfide reduction and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi243Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi246Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi250Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi254Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi291Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi294Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi297Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi301Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi580Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi583Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi586Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi590Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi609Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi612Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi615Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi619Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi147 – 170FADSequence analysisAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMethanogenesis
Ligand2Fe-2S, 4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-3076-MONOMER
MetaCyc:MONOMER-20159
UniPathwayiUPA00647; UER00700

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit ACurated (EC:1.8.7.31 Publication, EC:1.8.98.41 Publication)
Alternative name(s):
Coenzyme F420:CoB-CoM heterodisulfide,ferredoxin reductase subunit ACurated
Ferredoxin:CoB-CoM heterodisulfide reductase subunit ACurated
Gene namesi
Name:hdrA21 Publication
Ordered Locus Names:MA_2868
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001500561 – 793Ferredoxin/F(420)H(2)-dependent CoB-CoM heterodisulfide reductase subunit AAdd BLAST793

Proteomic databases

PRIDEiQ8TM02

Expressioni

Inductioni

Induced by growth on methanol or acetate.1 Publication

Interactioni

Subunit structurei

The ferredoxin/F(420)H2-dependent CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA2, HdrB2 and HdrC2.1 Publication

Protein-protein interaction databases

STRINGi188937.MA2868

Structurei

3D structure databases

ProteinModelPortaliQ8TM02
SMRiQ8TM02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 2644Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini282 – 3114Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
Domaini571 – 6004Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini601 – 6294Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Belongs to the HdrA family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG02235 Archaea
arCOG02476 Archaea
COG1148 LUCA
COG1908 LUCA
InParanoidiQ8TM02
KOiK03388
OMAiTAKHAML
OrthoDBiPOG093Z04QZ
PhylomeDBiQ8TM02

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR003813 FlpD
PfamiView protein in Pfam
PF00037 Fer4, 1 hit
PF13187 Fer4_9, 1 hit
PF02662 FlpD, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 4 hits

Sequencei

Sequence statusi: Complete.

Q8TM02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVYICHC GLNIAGVIDV SALEAMANEL EDVVLAREVQ FLCSDSGQEG
60 70 80 90 100
IIKDIKDNKL DRVVVAACSP RLHEKTFRHV MEKAGLNPYL MEMVNIREQC
110 120 130 140 150
SWVHADDPQM ATQKAFDLIR MGVAKARFLR ELSATNSKAS RNVLIIGGGV
160 170 180 190 200
AGIEAALNLA EAGFPVTMVE KESTIGGKMA LMNEVFPTND CSICVLAPKM
210 220 230 240 250
TEVQNHPNIT LYTYSEVTDI SGSVGKFHVR VKRKPRFVLE DKCKGCVDLC
260 270 280 290 300
SGVCPVEIEN PMNYGIGKTR AIYMPIPQSV PQVVLIDPDH CVGCGLCQLA
310 320 330 340 350
CPAEAVDYEQ KPEEIEFEAG AIIVSTGYQL FDASRKKEYG FGKYPDVITN
360 370 380 390 400
MQLERMLNSA GPTGGRVLVP STGEPPKSVA FIQCVGSRDK TVGNEYCSRV
410 420 430 440 450
CCMAALKNSQ MVKERYPDTD VTIHYIDIRA AGEMYEEYYT RTQEMGVDFI
460 470 480 490 500
RGKVAEVYSG EDGRPVVRFE NTLESSVEEE AHDLVVLSTG YEPTKAAEGI
510 520 530 540 550
GRMLNLARRP DRFFASAHPK MRPVDAPVSG VFLAGCASGP KEIQVSIAQG
560 570 580 590 600
SACASKVMQL LGTGELEADP MGAHVDPDKC IGCRTCVEVC KFGKISIENK
610 620 630 640 650
KAVVDEVSCY GCGDCSAACP VGAIQMRNFE NEQILAQVRE ATAHKSQCPF
660 670 680 690 700
IVAFLCNWCS YACADLTGMS RIRYPTNIRV IRTMCSARVN PEFVLEALKG
710 720 730 740 750
GADGVLVAGC RMDECHYIHG NFDAKKRMDI LKEVIKEIGL DPKRLRTLWI
760 770 780 790
SAAEGERFSN TINEFVKELE EIGPIGSEFK QECAVPGVEE VTQ
Length:793
Mass (Da):86,918
Last modified:June 1, 2002 - v1
Checksum:i80204B5433039032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM06247.1
RefSeqiWP_011022820.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM06247; AAM06247; MA_2868
GeneIDi1474765
KEGGimac:MA_2868

Similar proteinsi

Entry informationi

Entry nameiHDRA2_METAC
AccessioniPrimary (citable) accession number: Q8TM02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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